Derivation of Three-Dimensional Human Induced Pluripotent Stem Cell-Derived Vocal Fold Mucosa for Clinical and Pharmacological Applications

Healthy vocal fold mucosa is composed of two major cell types, non-keratinized stratified squamous epithelium and vocal fold fibroblasts. Although dysfunction of the epithelium may play a significant pathogenic role in vocal fold diseases, studies at the genetic and molecular level using primary epi...

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Bibliographic Details
Main Authors: Lungova, V. (Author), Thibeault, S.L (Author)
Format: Article
Language:English
Published: NLM (Medline) 2022
Subjects:
Online Access:View Fulltext in Publisher
LEADER 01811nam a2200205Ia 4500
001 10.1007-7651_2021_392
008 220630s2022 CNT 000 0 und d
020 |a 19406029 (ISSN) 
245 1 0 |a Derivation of Three-Dimensional Human Induced Pluripotent Stem Cell-Derived Vocal Fold Mucosa for Clinical and Pharmacological Applications 
260 0 |b NLM (Medline)  |c 2022 
520 3 |a Healthy vocal fold mucosa is composed of two major cell types, non-keratinized stratified squamous epithelium and vocal fold fibroblasts. Although dysfunction of the epithelium may play a significant pathogenic role in vocal fold diseases, studies at the genetic and molecular level using primary epithelial cells or models of human vocal fold mucosa have been significantly limited by the availability of relevant tissue types, poor growth, and heterogeneity of primary vocal fold epithelial cells. Here, we describe in vitro developmental differentiation of human induced pluripotent stem cells into vocal fold basal epithelial progenitors that were reseeded on collagen-fibroblast constructs to induce stratification and generate a three-dimensional model of human vocal fold mucosa. The engineered vocal fold mucosa represents physiologically relevant and clinically useful model that can be used as a tool for disease modeling and testing of therapeutic approaches for the treatment of laryngeal and VF inflammation. © 2021. Springer Science+Business Media, LLC. 
650 0 4 |a Developmental derivation 
650 0 4 |a Medical research 
650 0 4 |a Stem cells 
650 0 4 |a Tissue engineering 
650 0 4 |a Vocal fold mucosa 
700 1 0 |a Lungova, V.  |e author 
700 1 0 |a Thibeault, S.L.  |e author 
773 |t Methods in molecular biology (Clifton, N.J.) 
856 |z View Fulltext in Publisher  |u https://doi.org/10.1007/7651_2021_392