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10.1016-j.aca.2022.339846 |
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220706s2022 CNT 000 0 und d |
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|a 00032670 (ISSN)
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|a Target-triggered cascade signal amplification for sensitive electrochemical detection of SARS-CoV-2 with clinical application
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|b Elsevier B.V.
|c 2022
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|z View Fulltext in Publisher
|u https://doi.org/10.1016/j.aca.2022.339846
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|a The spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has led to the outbreak of the 2019 coronavirus (COVID-19) disease, which greatly challenges the global economy and health. Simple and sensitive diagnosis of COVID-19 at the early stage is important to prevent the spread of pandemics. Herein, we have proposed a target-triggered cascade signal amplification in this work for sensitive analysis of SARS-CoV-2 RNA. Specifically, the presence of SARS-CoV-2 RNA can trigger the catalytic hairpin assembly to generate plenty of DNA duplexes with free 3′-OH termini, which can be recognized and catalyzed by the terminal deoxynucleotidyl transferase (TdT) to generate long strand DNA. The prolonged DNA can absorb substantial Ru(NH3)6 3+ molecules via electrostatic interaction and produce an enhanced current response. The incorporation of catalytic hairpin assembly and TdT-mediated polymerization effectively lowers the detection limit to 45 fM, with a wide linear range from 0.1 pM to 3000 pM. Moreover, the proposed strategy possesses excellent selectivity to distinguish target RNA with single-base mismatched, three-base mismatched, and random sequences. Notably, the proposed electrochemical biosensor can be applied to analyze targets in complex circumstances containing 10% saliva, which implies its high stability and anti-interference. Moreover, the proposed strategy has been successfully applied to SARS CoV-2 RNA detection in clinical samples and may have the potential to be cultivated as an effective tool for COVID-19 diagnosis. © 2022 Elsevier B.V.
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|a Ammonia
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|a Amplification
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|a Biosensors
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|a Cascade signal amplification
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|a Cascade signal amplification
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|a Chemical detection
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|a Clinical application
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|a Coronavirus
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|a Coronaviruses
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|a COVID-19
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|a COVID-19
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|a Diagnosis
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|a DNA
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|a Electrochemical biosensor
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|a Electrochemical biosensor
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|a ELectrochemical detection
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|a Global health
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|a RNA
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|a SARS
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|a SARS-CoV-2 RNA
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|a Severe acute respiratory syndrome coronavirus
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|a Severe acute respiratory syndrome coronavirus 2 RNA
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|a Signal amplifications
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|a Deng, Y.
|e author
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|a Li, G.
|e author
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|a Li, J.
|e author
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|a Peng, Y.
|e author
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|a Wang, L.
|e author
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|a Wang, M.
|e author
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|a Xiang, L.
|e author
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|a Yang, J.
|e author
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|a Zhou, T.
|e author
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|t Analytica Chimica Acta
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