Immobilisation of cyclodextrin glucanotransferase into polyvinyl alcohol (PVA) nanofibres via electrospinning
Immobilisation of cyclodextrin glucanotransferase (CGTase) on nanofibres was demonstrated. CGTase solution (1% v/v) and PVA (8 wt%) solution were mixed followed by electrospinning (-9 kV, 3 h). CGTase/PVA nanofibres with an average diameter of 176 ± 46 nm were successfully produced. The nanofibres t...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier B.V.
2016
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| Subjects: | |
| Online Access: | View Fulltext in Publisher View in Scopus |
| LEADER | 02023nam a2200373Ia 4500 | ||
|---|---|---|---|
| 001 | 10.1016-j.btre.2016.03.003 | ||
| 008 | 220120s2016 CNT 000 0 und d | ||
| 020 | |a 2215017X (ISSN) | ||
| 245 | 1 | 0 | |a Immobilisation of cyclodextrin glucanotransferase into polyvinyl alcohol (PVA) nanofibres via electrospinning |
| 260 | 0 | |b Elsevier B.V. |c 2016 | |
| 520 | 3 | |a Immobilisation of cyclodextrin glucanotransferase (CGTase) on nanofibres was demonstrated. CGTase solution (1% v/v) and PVA (8 wt%) solution were mixed followed by electrospinning (-9 kV, 3 h). CGTase/PVA nanofibres with an average diameter of 176 ± 46 nm were successfully produced. The nanofibres that consist of immobilised CGTase were crosslinked with glutaraldehyde vapour. A CGTase/PVA film made up from the same mixture and treated the same way was used as a control experiment. The immobilised CGTase on nanofibres showed superior performance with nearly a 2.5 fold higher enzyme loading and 31% higher enzyme activity in comparison with the film. © 2016 Elsevier B.V. All rights reserved. | |
| 650 | 0 | 4 | |a Article |
| 650 | 0 | 4 | |a CGTase |
| 650 | 0 | 4 | |a cross linking |
| 650 | 0 | 4 | |a Cyclodextrins |
| 650 | 0 | 4 | |a cyclomaltodextrin glucanotransferase |
| 650 | 0 | 4 | |a electrospinning |
| 650 | 0 | 4 | |a Electrospinning |
| 650 | 0 | 4 | |a enzyme activity |
| 650 | 0 | 4 | |a enzyme immobilization |
| 650 | 0 | 4 | |a glutaraldehyde |
| 650 | 0 | 4 | |a Immobilisation |
| 650 | 0 | 4 | |a nanofiber |
| 650 | 0 | 4 | |a Nanofibres |
| 650 | 0 | 4 | |a polyvinyl alcohol |
| 700 | 1 | 0 | |a Abu Bakar, N.F. |e author |
| 700 | 1 | 0 | |a Gen, M. |e author |
| 700 | 1 | 0 | |a Lenggoro, I.W. |e author |
| 700 | 1 | 0 | |a Mokhtar, M.N. |e author |
| 700 | 1 | 0 | |a Naim, M.N. |e author |
| 700 | 1 | 0 | |a Saallah, S. |e author |
| 773 | |t Biotechnology Reports |x 2215017X (ISSN) |g 10, 44-48 | ||
| 856 | |z View Fulltext in Publisher |u https://doi.org/10.1016/j.btre.2016.03.003 | ||
| 856 | |z View in Scopus |u https://www.scopus.com/inward/record.uri?eid=2-s2.0-84961784041&doi=10.1016%2fj.btre.2016.03.003&partnerID=40&md5=84369928ca74779998bf7eabfbb59fcb | ||