Surface enhanced Raman scattering (SERS)-active bacterial detection by Layer-by-Layer (LbL) assembly all-nanoparticle microcapsules

• Main Authors: Khalenkow, D. (Author), Lapanje, A. (Author), Li, J. (Author), Parakhonskiy, B.V (Author), Skirtach, A.G (Author), Volodkin, D. (Author)
• Format: Article
• Language: English
• Published: Elsevier B.V. 2022
• Subjects: Bacterial; Bacterial detection; Calcium carbonate; Capsule; Capsules; Deposition; Drug delivery; Enhanced Raman scattering; Layer by layer; Layer-by-layer approaches; Layer-by-layer assemblies; LbL; Macromolecules; Microcapsules; Microstructure; Multilayer; Nanoparticles; Polyelectrolyte; Polyelectrolytes; Raman scattering; SERS; Surface enhanced Raman; Surface enhanced raman scattering; Surface scattering
• Online Access: View Fulltext in Publisher

 Polymeric microcapsules composed by the Layer-by-Layer (LbL) approach have been used for various applications including drug delivery into cells and in vivo, conducting enzyme catalyzed reactions, performing sensoric functions. Typically, LbL-assembled microcapsules have been formulated via alternating deposition of positively and negatively charged polyelectrolytes onto sacrificial templates or so-called cores. In this work, we extend the LbL assembly to produce microcapsules solely based on nanoparticles instead of polymers. We have identified that 5 layers of nanoparticles is the minimum number of layers for a stable assembly of capsules on calcium carbonate templates. Subsequently, these capsules comprised of nanoparticles were applied as Surface Enhanced Raman Scattering (SERS) platform, where the nanoparticle-based shell of capsules is shown to enable SERS of both solutes and macromolecular structures such as bacterial cells. © 2022 Elsevier B.V.