A peptide cross-linked polyacrylamide hydrogel for the detection of human neutrophil elastase
Polyacrylamide hydrogel films cross-linked with the peptide sequence AAPVAAK were synthesised on piezoelectric quartz crystals. Degradation of the hydrogel films was monitored using a combination of quartz crystal microbalance (QCM) and electrochemical impedance measurements. The films were shown to...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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| Online Access: | View Fulltext in Publisher View in Scopus |
| LEADER | 02424nam a2200481Ia 4500 | ||
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| 001 | 10.1016-j.electacta.2009.03.067 | ||
| 008 | 220112s2009 CNT 000 0 und d | ||
| 020 | |a 00134686 (ISSN) | ||
| 245 | 1 | 0 | |a A peptide cross-linked polyacrylamide hydrogel for the detection of human neutrophil elastase |
| 856 | |z View Fulltext in Publisher |u https://doi.org/10.1016/j.electacta.2009.03.067 | ||
| 856 | |z View in Scopus |u https://www.scopus.com/inward/record.uri?eid=2-s2.0-65749095562&doi=10.1016%2fj.electacta.2009.03.067&partnerID=40&md5=57eb07cf67522c814e5223c255727173 | ||
| 520 | 3 | |a Polyacrylamide hydrogel films cross-linked with the peptide sequence AAPVAAK were synthesised on piezoelectric quartz crystals. Degradation of the hydrogel films was monitored using a combination of quartz crystal microbalance (QCM) and electrochemical impedance measurements. The films were shown to degrade in the presence of human neutrophil elastase (HNE). The rate of degradation was directly related to the enzyme activity making the system suitable for the detection of HNE in the activity range from 0.72 to 30 U mL-1. The films were not affected by non-specific adsorption. Film degradation was only accompanied by changes in the QCM signal, while no significant impedance change was observed during degradation. © 2009 Elsevier Ltd. All rights reserved. | |
| 650 | 0 | 4 | |a Adsorption |
| 650 | 0 | 4 | |a Amines |
| 650 | 0 | 4 | |a Degradation |
| 650 | 0 | 4 | |a Electrochemical impedance measurements |
| 650 | 0 | 4 | |a Enzymatic degradation |
| 650 | 0 | 4 | |a Enzyme activity |
| 650 | 0 | 4 | |a Fiber optic chemical sensors |
| 650 | 0 | 4 | |a Film degradation |
| 650 | 0 | 4 | |a Human neutrophil elastase |
| 650 | 0 | 4 | |a Hydrogel films |
| 650 | 0 | 4 | |a Hydrogels |
| 650 | 0 | 4 | |a Impedance |
| 650 | 0 | 4 | |a Nonspecific adsorption |
| 650 | 0 | 4 | |a Oxide minerals |
| 650 | 0 | 4 | |a Peptide cross-linked hydrogel |
| 650 | 0 | 4 | |a Peptide sequences |
| 650 | 0 | 4 | |a Piezoelectric devices |
| 650 | 0 | 4 | |a Piezoelectric quartz crystals |
| 650 | 0 | 4 | |a Piezoelectricity |
| 650 | 0 | 4 | |a Polyacrylamide |
| 650 | 0 | 4 | |a Polyacrylamide hydrogels |
| 650 | 0 | 4 | |a Polyacrylates |
| 650 | 0 | 4 | |a Quartz |
| 650 | 0 | 4 | |a Quartz crystal microbalance |
| 650 | 0 | 4 | |a Quartz crystal microbalances |
| 700 | 1 | 0 | |a Hunter, C.A. |e author |
| 700 | 1 | 0 | |a Kamarun, D. |e author |
| 700 | 1 | 0 | |a Krause, S. |e author |
| 700 | 1 | 0 | |a Milanesi, L. |e author |
| 700 | 1 | 0 | |a Zheng, X. |e author |
| 773 | |t Electrochimica Acta | ||