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04007nam a2200949Ia 4500 |
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10.1016-j.ijbiomac.2021.04.136 |
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220427s2021 CNT 000 0 und d |
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|a 01418130 (ISSN)
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|a Isolation and modification of nano-scale cellulose from organosolv-treated birch through the synergistic activity of LPMO and endoglucanases
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|b Elsevier B.V.
|c 2021
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|z View Fulltext in Publisher
|u https://doi.org/10.1016/j.ijbiomac.2021.04.136
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|a Nanocellulose isolation from lignocellulose is a tedious and expensive process with high energy and harsh chemical requirements, primarily due to the recalcitrance of the substrate, which otherwise would have been cost-effective due to its abundance. Replacing the chemical steps with biocatalytic processes offers opportunities to solve this bottleneck to a certain extent due to the enzymes substrate specificity and mild reaction chemistry. In this work, we demonstrate the isolation of sulphate-free nanocellulose from organosolv pretreated birch biomass using different glycosyl-hydrolases, along with accessory oxidative enzymes including a lytic polysaccharide monooxygenase (LPMO). The suggested process produced colloidal nanocellulose suspensions (ζ-potential −19.4 mV) with particles of 7–20 nm diameter, high carboxylate content and improved thermostability (To = 301 °C, Tmax = 337 °C). Nanocelluloses were subjected to post-modification using LPMOs of different regioselectivity. The sample from chemical route was the least favorable for LPMO to enhance the carboxylate content, while that from the C1-specific LPMO treatment showed the highest increase in carboxylate content. © 2021
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|a Article
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|a atomic force microscopy
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|a Betula
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|a Betula pendula
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|a biomass
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|a Biomass
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|a birch
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|a budding yeast
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|a carboxylic acid
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|a cellulase
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|a Cellulase
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|a cellulose
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|a Cellulose
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|a controlled study
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|a enzyme specificity
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|a enzymology
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|a genetics
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|a glucan synthase
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|a hydrolysis
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|a Hydrolysis
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|a hydrophobic interaction chromatography
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|a isolation and purification
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|a Komagataella pastoris
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|a laccase
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|a Laccase
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|a lignin
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|a Lignin
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|a lignocellulose
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|a LPMO biocatalysis
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|a lytic polysaccharide monooxygenase
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|a metabolism
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|a mixed function oxidase
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|a Mixed Function Oxygenases
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|a molecular cloning
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|a Myceliophthora thermophila
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|a nanocellulose
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|a Nanocellulose
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|a nanofiber
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|a Nanofibers
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|a nonhuman
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|a organic solvent
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|a organosolv
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|a Phanerochaete
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|a Phanerochaete
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|a Post-treatment modification/functionalization
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|a regioselectivity
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|a Saccharomycetales
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|a scanning electron microscopy
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|a Sordariales
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|a Sordariales
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|a Substrate Specificity
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|a suspension
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|a thermogravimetry
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|a thermostability
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|a unclassified drug
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|a unspecific monooxygenase
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|a X ray photoemission spectroscopy
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|a xylan 1,4 beta xylosidase
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|a Xylosidases
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|a zeta potential
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|a Christakopoulos, P.
|e author
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|a Karnaouri, A.
|e author
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|a Liu, B.
|e author
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|a Mathew, A.P.
|e author
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|a Matsakas, L.
|e author
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|a Muraleedharan, M.N.
|e author
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|a Piatkova, M.
|e author
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|a Rova, U.
|e author
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|a Ruiz-Caldas, M.-X.
|e author
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|t International Journal of Biological Macromolecules
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