Exploring molecular mechanism of bone-forming capacity of Eurycoma longifolia: Evidence of enhanced expression of bone-related biomarkers
Background: Among the numerous well-documented medicinal herbs, Eurycoma longifolia (EL) has gained remarkable recognition due to its promising efficacy of stimulating bone formation in androgen-deficient osteoporosis. Though numerous animal studies have explored the bone-forming capacity of EL, the...
Main Authors: | , , , |
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Format: | Article |
Language: | English |
Published: |
Elsevier B.V.
2018
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Online Access: | View Fulltext in Publisher View in Scopus |
LEADER | 04005nam a2200625Ia 4500 | ||
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001 | 10.1016-j.jaim.2017.04.005 | ||
008 | 220120s2018 CNT 000 0 und d | ||
020 | |a 09759476 (ISSN) | ||
245 | 1 | 0 | |a Exploring molecular mechanism of bone-forming capacity of Eurycoma longifolia: Evidence of enhanced expression of bone-related biomarkers |
260 | 0 | |b Elsevier B.V. |c 2018 | |
490 | 1 | |t Journal of Ayurveda and Integrative Medicine | |
650 | 0 | 4 | |a alkaline phosphatase |
650 | 0 | 4 | |a androgen receptor |
650 | 0 | 4 | |a animal cell |
650 | 0 | 4 | |a Article |
650 | 0 | 4 | |a biological marker |
650 | 0 | 4 | |a Bone formation |
650 | 0 | 4 | |a bone morphogenetic protein |
650 | 0 | 4 | |a bone morphogenetic protein 2 |
650 | 0 | 4 | |a cell differentiation |
650 | 0 | 4 | |a cell growth |
650 | 0 | 4 | |a cell structure |
650 | 0 | 4 | |a cell viability |
650 | 0 | 4 | |a collagen synthesis |
650 | 0 | 4 | |a collagen type 1 |
650 | 0 | 4 | |a comparative study |
650 | 0 | 4 | |a controlled study |
650 | 0 | 4 | |a enzyme activity |
650 | 0 | 4 | |a Eurycoma |
650 | 0 | 4 | |a Eurycoma longifolia |
650 | 0 | 4 | |a in vitro study |
650 | 0 | 4 | |a MC3T3-E1 cell line |
650 | 0 | 4 | |a mineralization |
650 | 0 | 4 | |a Molecular mechanism |
650 | 0 | 4 | |a mouse |
650 | 0 | 4 | |a nonhuman |
650 | 0 | 4 | |a osteoblast |
650 | 0 | 4 | |a osteocalcin |
650 | 0 | 4 | |a osteopontin |
650 | 0 | 4 | |a osteoporosis |
650 | 0 | 4 | |a Osteoporosis |
650 | 0 | 4 | |a phase contrast microscopy |
650 | 0 | 4 | |a priority journal |
650 | 0 | 4 | |a testosterone |
650 | 0 | 4 | |a transcription factor RUNX2 |
650 | 0 | 4 | |a transforming growth factor beta1 |
650 | 0 | 4 | |a upregulation |
856 | |z View Fulltext in Publisher |u https://doi.org/10.1016/j.jaim.2017.04.005 | ||
856 | |z View in Scopus |u https://www.scopus.com/inward/record.uri?eid=2-s2.0-85044790498&doi=10.1016%2fj.jaim.2017.04.005&partnerID=40&md5=1a5f464743c4d229d305b66c05ded6f0 | ||
520 | 3 | |a Background: Among the numerous well-documented medicinal herbs, Eurycoma longifolia (EL) has gained remarkable recognition due to its promising efficacy of stimulating bone formation in androgen-deficient osteoporosis. Though numerous animal studies have explored the bone-forming capacity of EL, the exact mechanism was yet to be explored. Objective(s): The present study was aimed to investigate the mechanism of bone-forming capacity of EL using MC3T3-E1 as an in vitro osteoblastic model. Materials and methods: The cell differentiation capacity of EL was investigated by evaluating cell growth, alkaline phosphatase (ALP) activity, collagen deposition and mineralization. Taken together, time-mannered expression of bone-related mediators which include bone morphogenic protein-2 (BMP-2), ALP, runt-related transcription factor-2 (Runx-2), osteocalcin (OCN), type I collagen, osteopontin (OPN), transforming growth factor-β1 (TGF-β1) and androgen receptor (AR) were measured to comprehend bone-forming mechanism of EL. Results: Results demonstrated a superior cell differentiation efficacy of EL (particularly at a dose of 25 μg/mL) that was evidenced by dramatically increased cell growth, higher ALP activity, collagen deposition and mineralization compared to the testosterone. Results analysis of the bone-related protein biomarkers indicated that the expression of these mediators was well-regulated in EL-treated cell cultures compared to the control groups. These findings revealed potential molecular mechanism of EL for the prevention and treatment of male osteoporosis. Conclusion: The resulting data suggested that EL exhibited superior efficacy in stimulating bone formation via up-regulating the expression of various mitogenic proteins and thus can be considered as a potential natural alternative therapy for the treatment of osteoporosis. © 2017 Transdisciplinary University, Bangalore and World Ayurveda Foundation | |
700 | 1 | 0 | |a Hussain, Z. |e author |
700 | 1 | 0 | |a Mohamed, I.N. |e author |
700 | 1 | 0 | |a Shuid, A.N. |e author |
700 | 1 | 0 | |a Thu, H.E. |e author |
773 | |t Journal of Ayurveda and Integrative Medicine |