In situ effect of Tooth Mousse containing CPP-ACP on human enamel subjected to in vivo acid attacks

• Main Authors: Attin, T. (Author), Cheng, H. (Author), Jiang, N.-W (Author), Ye, X.-Y (Author), Yu, H. (Author), Zheng, H.-Y (Author)
• Format: Article
• Language: English
• Published: Elsevier Ltd 2018
• Subjects: acid; Acids; carbonated beverage; Carbonated Beverages; casein; casein phosphopeptide-amorphous calcium phosphate nanocomplex; Caseins; controlled study; crossover procedure; Cross-Over Studies; Demineralization; Dental Enamel; dental procedure; drug effect; enamel; Enamel; Hardness; human; Humans; randomized controlled trial; tooth disease; Tooth erosion; Tooth Erosion; Tooth Remineralization
• Online Access: View Fulltext in Publisher

 Objective: This in situ study aimed to evaluate the protective effect of Tooth Mousse (GC) containing casein phosphopeptide-amorphous calcium phosphate (CPP-ACP) on human enamel erosion and to compare the difference in erosion between the anteriorly and posteriorly positioned human enamel. Methods: This study used a 2-treatment (7 days each) crossover design with 12 healthy volunteers wearing intraoral appliances. Each appliance contained 4 human enamel specimens positioned on the buccal surfaces of the volunteers’ maxillary central incisors and first molars. The specimens were intraorally treated withTooth Mousse (CPP-ACP group) or deionized water (control group) for 3 min and then exposed to in vivo acid attacks by rinsing with 150 ml of a cola drink (4 × 5 min/day). The surface microhardness (SMH) of the specimens was measured and used to calculate the percentage of SMH loss (%SMHl). Erosion effect on enamel was also investigated by scanning electron microscopy (n = 4) at the end of study. The data were statistically analysed using two-way analysis of variance (ANOVA) and Tukey's test at a level of P < 0.05. Results: A significant decrease in %SMHl was observed for the specimens of CPP-ACP group compared to that for the controls (P = 0.007). The specimens positioned posteriorly exhibited a significantly lower %SMHl than those positioned anteriorly (P = 0.033). Samples of CPP-ACP group showed fewer etching patterns than those of the control group. Conclusions: In this in situ model, application of Tooth Mousse containing CPP-ACP before erosion reduced the %SMHl of human enamel. Enamel located in different positions showed different patterns of erosion. Clinical significance: Application of Tooth Mousse containing CPP-ACP could be considered as a suitable preventive strategy against enamel erosion. ClinicalTrials.gov Identifier: NCT03426150. © 2018 Elsevier Ltd