Potentiated antimicrobial blue light killing of methicillin resistant Staphylococcus aureus by pyocyanin

As antimicrobial resistance continues to threaten the efficacy of conventional antibiotic therapy, it is paramount that we investigate innovative approaches to treat infectious diseases. In this study, we investigated the antimicrobial capabilities of the innovative combination of antimicrobial blue...

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Bibliographic Details
Main Authors: Dai, T. (Author), Leanse, L.G (Author), Zeng, X. (Author)
Format: Article
Language:English
Published: Elsevier B.V. 2021
Subjects:
Online Access:View Fulltext in Publisher
LEADER 03958nam a2200769Ia 4500
001 10.1016-j.jphotobiol.2020.112109
008 220427s2021 CNT 000 0 und d
020 |a 10111344 (ISSN) 
245 1 0 |a Potentiated antimicrobial blue light killing of methicillin resistant Staphylococcus aureus by pyocyanin 
260 0 |b Elsevier B.V.  |c 2021 
856 |z View Fulltext in Publisher  |u https://doi.org/10.1016/j.jphotobiol.2020.112109 
520 3 |a As antimicrobial resistance continues to threaten the efficacy of conventional antibiotic therapy, it is paramount that we investigate innovative approaches to treat infectious diseases. In this study, we investigated the antimicrobial capabilities of the innovative combination of antimicrobial blue light (aBL; 405 nm wavelength) with the Pseudomonas aeruginosa pigment pyocyanin against methicillin resistant Staphylococcus aureus (MSRA. We explored the effects of different radiant exposures of aBL and increasing concentrations of pyocyanin against planktonic cells and those within biofilms. In addition, we investigated the effect of the aBL/pyocyanin on the endogenous staphyloxanthin pigment, as well as the role of hydrogen peroxide and singlet oxygen scavenging in the efficacy of this combination. Lastly, we investigated the potential for the aBL/pyocyanin to reduce the MRSA burden within a proof-of-principle mouse abrasion infection model. We found pyocyanin to be a powerful potentiator of aBL activity under all in vitro conditions tested. In addition, we serendipitously discovered the capability of the aBL/pyocyanin combination to bleach staphyloxanthin within colonies of MRSA. Furthermore, we established that singlet oxygen is an important mediator during combined aBL/pyocyanin exposure. Moreover, we found that the combination of aBL and pyocyanin could significantly reduce the viability of MRSA within a proof-of-principle early onset MRSA skin abrasion infection. Exposure to the treatment did not have deleterious effects on skin tissue. In conclusion, the combination of aBL and pyocyanin represents a potentially powerful therapeutic modality for the treatment of infections caused by MRSA. © 2020 Elsevier B.V. 
650 0 4 |a abrasion 
650 0 4 |a animal 
650 0 4 |a Animals 
650 0 4 |a antimicrobial activity 
650 0 4 |a Antimicrobial blue light 
650 0 4 |a Antimicrobial resistance 
650 0 4 |a Article 
650 0 4 |a bacterial colonization 
650 0 4 |a bacterial infection 
650 0 4 |a bacterial load 
650 0 4 |a biofilm 
650 0 4 |a Biofilms 
650 0 4 |a blue light 
650 0 4 |a concentration (parameter) 
650 0 4 |a connective tissue 
650 0 4 |a controlled study 
650 0 4 |a dose response 
650 0 4 |a Dose-Response Relationship, Drug 
650 0 4 |a drug effect 
650 0 4 |a drug exposure 
650 0 4 |a hydrogen peroxide 
650 0 4 |a in vitro study 
650 0 4 |a light 
650 0 4 |a Light 
650 0 4 |a methicillin resistant Staphylococcus aureus 
650 0 4 |a methicillin resistant Staphylococcus aureus 
650 0 4 |a Methicillin-Resistant Staphylococcus aureus 
650 0 4 |a Mice 
650 0 4 |a microbial viability 
650 0 4 |a Microbial Viability 
650 0 4 |a microbiology 
650 0 4 |a mouse 
650 0 4 |a MRSA 
650 0 4 |a nonhuman 
650 0 4 |a physiology 
650 0 4 |a plankton 
650 0 4 |a priority journal 
650 0 4 |a proof of concept 
650 0 4 |a Pseudomonas aeruginosa 
650 0 4 |a Pyocyanin 
650 0 4 |a pyocyanine 
650 0 4 |a pyocyanine 
650 0 4 |a Pyocyanine 
650 0 4 |a radiation response 
650 0 4 |a singlet oxygen 
650 0 4 |a skin 
650 0 4 |a Skin 
650 0 4 |a staphyloxanthin 
650 0 4 |a unclassified drug 
650 0 4 |a Wound infection 
650 0 4 |a xanthine 
700 1 |a Dai, T.  |e author 
700 1 |a Leanse, L.G.  |e author 
700 1 |a Zeng, X.  |e author 
773 |t Journal of Photochemistry and Photobiology B: Biology