|
|
|
|
| LEADER |
03061nam a2200565Ia 4500 |
| 001 |
10.1016-j.jphotobiol.2021.112150 |
| 008 |
220427s2021 CNT 000 0 und d |
| 020 |
|
|
|a 10111344 (ISSN)
|
| 245 |
1 |
0 |
|a Structural membrane changes induced by pulsed blue light on methicillin-resistant Staphylococcus aureus (MRSA)
|
| 260 |
|
0 |
|b Elsevier B.V.
|c 2021
|
| 856 |
|
|
|z View Fulltext in Publisher
|u https://doi.org/10.1016/j.jphotobiol.2021.112150
|
| 520 |
3 |
|
|a Background: In a recent study we showed that blue light inactivates methicillin-resistant Staphylococcus aureus (MRSA) by perturbing, depolarizing, and disrupting its cell membrane. Purpose: The current study presents visual evidence that the observed biochemical changes also result in cell metabolic changes and structural alteration of the cell membrane. Methods: Cultures of MRSA were treated with 450 nm pulsed blue light (PBL) at 3 mW/cm2 irradiance, using a sub lethal dose of 2.7 J/cm2 radiant exposure three times at 30-min intervals. Following 24 h incubation at 37 °C, irradiated colonies and control non-irradiated colonies were processed for light and transmission electron microscopy. Results: The images obtained revealed three major effects of PBL; (1) disruption of MRSA cell membrane, (2) alteration of membrane structure, and (3) disruption of cell replication. Conclusion: These signs of bacterial inactivation at a dose deliberately selected to be sub-lethal supports our previous finding that rapid depolarization of bacterial cell membrane and disruption of cellular function comprise another mechanism underlying photo-inactivation of bacteria. Further, it affirms the potency of PBL. © 2021 The Authors
|
| 650 |
0 |
4 |
|a Article
|
| 650 |
0 |
4 |
|a bacterial cell wall
|
| 650 |
0 |
4 |
|a bacterial count
|
| 650 |
0 |
4 |
|a bacterial membrane
|
| 650 |
0 |
4 |
|a blue light
|
| 650 |
0 |
4 |
|a cell culture technique
|
| 650 |
0 |
4 |
|a Cell Culture Techniques
|
| 650 |
0 |
4 |
|a cell division
|
| 650 |
0 |
4 |
|a cell membrane
|
| 650 |
0 |
4 |
|a Cell Membrane
|
| 650 |
0 |
4 |
|a cell membrane depolarization
|
| 650 |
0 |
4 |
|a cell metabolism
|
| 650 |
0 |
4 |
|a Colony Count, Microbial
|
| 650 |
0 |
4 |
|a controlled study
|
| 650 |
0 |
4 |
|a Dose-Response Relationship, Radiation
|
| 650 |
0 |
4 |
|a Electron microscopy
|
| 650 |
0 |
4 |
|a light
|
| 650 |
0 |
4 |
|a Light
|
| 650 |
0 |
4 |
|a membrane damage
|
| 650 |
0 |
4 |
|a metabolism
|
| 650 |
0 |
4 |
|a methicillin resistant Staphylococcus aureus
|
| 650 |
0 |
4 |
|a methicillin resistant Staphylococcus aureus
|
| 650 |
0 |
4 |
|a Methicillin-Resistant Staphylococcus aureus
|
| 650 |
0 |
4 |
|a microbial viability
|
| 650 |
0 |
4 |
|a Microbial Viability
|
| 650 |
0 |
4 |
|a MRSA
|
| 650 |
0 |
4 |
|a nonhuman
|
| 650 |
0 |
4 |
|a Photobiomodulation
|
| 650 |
0 |
4 |
|a priority journal
|
| 650 |
0 |
4 |
|a Pulsed blue light
|
| 650 |
0 |
4 |
|a radiation response
|
| 650 |
0 |
4 |
|a transmission electron microscopy
|
| 700 |
1 |
|
|a Bowman, C.
|e author
|
| 700 |
1 |
|
|a Bumah, V.V.
|e author
|
| 700 |
1 |
|
|a Cortez, P.
|e author
|
| 700 |
1 |
|
|a Enwemeka, C.S.
|e author
|
| 700 |
1 |
|
|a Niesman, I.R.
|e author
|
| 773 |
|
|
|t Journal of Photochemistry and Photobiology B: Biology
|
