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01488nam a2200265Ia 4500 |
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10.1016-j.xpro.2022.101370 |
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220706s2022 CNT 000 0 und d |
| 020 |
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|a 26661667 (ISSN)
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| 245 |
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|a Measurement of cerebral oxygen pressure in living mice by two-photon phosphorescence lifetime microscopy
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| 260 |
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|b Cell Press
|c 2022
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|z View Fulltext in Publisher
|u https://doi.org/10.1016/j.xpro.2022.101370
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|a The ability to quantify partial pressure of oxygen (pO2) is of primary importance for studies of metabolic processes in health and disease. Here, we present a protocol for imaging of oxygen distributions in tissue and vasculature of the cerebral cortex of anesthetized and awake mice. We describe in vivo two-photon phosphorescence lifetime microscopy (2PLM) of oxygen using the probe Oxyphor 2P. This minimally invasive protocol outperforms existing approaches in terms of accuracy, resolution, and imaging depth. For complete details on the use and execution of this protocol, please refer to Esipova et al. (2019). © 2022 The Authors
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|a Metabolism
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|a Microscopy
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|a Model Organisms
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|a Molecular/Chemical Probes
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|a Neuroscience
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|a Condrau, J.
|e author
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|a Erlebach, E.
|e author
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|a Ravotto, L.
|e author
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|a Troxler, T.
|e author
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|a Vinogradov, S.A.
|e author
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|a Weber, B.
|e author
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|a Wyss, M.T.
|e author
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| 773 |
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|t STAR Protocols
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