The importance of biofilm formation for cultivation of a Micrarchaeon and its interactions with its Thermoplasmatales host

• Main Authors: Bharat, T.A.M (Author), Bunk, B. (Author), Dombrowski, N. (Author), Gescher, J. (Author), Gfrerer, S. (Author), Hiller, K. (Author), Krause, S. (Author), Kuhlicke, U. (Author), Neu, T.R (Author), Rachel, R. (Author), Reuse, C. (Author), Schmidt-Hohagen, K. (Author), Spang, A. (Author), Spröer, C. (Author), Villanueva, L. (Author), von Kügelgen, A. (Author)
• Format: Article
• Language: English
• Published: Nature Research 2022
• Subjects: Archaea; archaeal genome; archaeon; biofilm; Biofilms; biology; cell component; cultivation; endogenous growth; genetics; Genome, Archaeal; growth; metabolism; phylogeny; Phylogeny; physiology; symbiont; Thermoplasmales; Thermoplasmatales
• Online Access: View Fulltext in Publisher
• ISBN: 20411723 (ISSN)
• DOI: 10.1038/s41467-022-29263-y

Micrarchaeota is a distinctive lineage assigned to the DPANN archaea, which includes poorly characterised microorganisms with reduced genomes that likely depend on interactions with hosts for growth and survival. Here, we report the enrichment of a stable co-culture of a member of the Micrarchaeota (Ca. Micrarchaeum harzensis) together with its Thermoplasmatales host (Ca. Scheffleriplasma hospitalis), as well as the isolation of the latter. We show that symbiont-host interactions depend on biofilm formation as evidenced by growth experiments, comparative transcriptomic analyses and electron microscopy. In addition, genomic, metabolomic, extracellular polymeric substances and lipid content analyses indicate that the Micrarchaeon symbiont relies on the acquisition of metabolites from its host. Our study of the cell biology and physiology of a Micrarchaeon and its host adds to our limited knowledge of archaeal symbioses. © 2022, The Author(s).