In vitro chondrogenesis transformation study of mouse dental pulp stem cells

• Main Authors: Abdul Razak, M. (Author), Kermani, S. (Author), Megat Abdul Wahab, R. (Author), Senafi, S. (Author), Zainal Ariffin, S.H (Author), Zainal Ariffin, Z. (Author)
• Format: Article
• Language: English
• Subjects: activated leukocyte cell adhesion molecule; alkaline phosphatase; animal; animal cell; Animals; antigen expression; article; Base Sequence; cartilage cell; CD146 antigen; cell culture; cell differentiation; cell structure; cell viability; chemical analysis; chondrogenesis; Chondrogenesis; controlled study; cytology; Dental Pulp; dental pulp stem cell; DNA Primers; enzyme activity; in vitro study; mesenchymal stem cell; Mice; mouse; nonhuman; nucleotide sequence; primer DNA; proteoglycan; Reverse Transcriptase Polymerase Chain Reaction; reverse transcription polymerase chain reaction; stem cell; Stem Cells; tolonium chloride; tooth pulp
• Online Access: View Fulltext in Publisher; View in Scopus

 A major challenge in the application of mesenchymal stem cells in cartilage reconstruction is that whether the cells are able to differentiate into fully mature chondrocytes before grafting. The aim of this study was to isolate mouse dental pulp stem cells (DPSC) and differentiate them into chondrocytes. For this investigation, morphological, molecular, and biochemical analyses for differentiated cells were used. To induce the chondrocyte differentiation, DPSC were cultured in chondrogenic medium (Zen-Bio, Inc.). Based on morphological analyses using toluidine blue staining, proteoglycan products appear in DPSC after 21 days of chondrocyte induction. Biochemical analyses in differentiated group showed that alkaline phosphatase activity was significantly increased at day 14 as compared to control (P < 0.05). Cell viability analyses during the differentiation to chondrocytes also showed that these cells were viable during differentiation. However, after the 14th day of differentiation, there was a significant decrease (P < 0.05) in the viability proportion among differentiated cells as compared to the control cells. In RT-PCR molecular analyses, mouse DPSC expressed Cd146 and Cd166 which indicated that these cells belong to mesenchymal stem cells. Coll I and Coll II markers showed high expression after 14 and 21 days, respectively. In conclusion, this study showed that DPSC successfully differentiated into chondrocytes. © 2012 Shahrul Hisham Zainal Ariffin et al.