Lnc-NTF3-5 promotes osteogenic differentiation of maxillary sinus membrane stem cells via sponging miR-93-3p

• Main Authors: Chen, L.-L (Author), Chen, S.-L (Author), Peng, W. (Author), Wang, J. (Author), Weng, J.-Q (Author), Zhu, S.-X (Author)
• Format: Article
• Language: English
• Published: Blackwell Publishing Ltd 2018
• Subjects: alkaline phosphatase; Alkaline Phosphatase; bone development; cell culture; cell differentiation; Cell Differentiation; Cells, Cultured; Core Binding Factor Alpha 1 Subunit; cytology; human; Humans; long non-coding RNAs; long untranslated RNA; maxillary sinus; Maxillary Sinus; maxillary sinus membrane stem cells; Metabolic Networks and Pathways; metabolism; microRNA; microRNAs; MicroRNAs; MIRN93 microRNA, human; multipotent stem cell; Multipotent Stem Cells; osteoblast; Osteoblasts; Osteogenesis; osteogenic differentiation; physiology; real time polymerase chain reaction; Real-Time Polymerase Chain Reaction; RNA, Long Noncoding; Sp7 protein, human; Sp7 Transcription Factor; transcription factor osterix; transcription factor RUNX2
• Online Access: View Fulltext in Publisher

 Background: The function and the mechanism of long non-coding RNAs (lncRNAs) on the osteogenic differentiation of maxillary sinus membrane stem cells (MSMSCs) remain largely unknown. Materials and Methods: The expression of lnc-NTF3-5 and Runt-related transcription factor 2 (RUNX2), Osterix (OSX), and Alkaline Phosphatase (ALP) was examined by quantitative real-time PCR (qRT-PCR) in MSMSCs during the process osteogenic differentiation. Then the function of lnc-NTF3-5 was evaluated by loss- and gain-of-function techniques, as well as qRT-PCR, western blot, and Alizarin Red staining. In addition, the microRNAs (miRNAs) sponge potential of lnc-NTF3-5 was assessed through RNA immunoprecipitation, dual luciferase reporter assay, and in vivo ectopic bone formation. Results: Lnc-NTF3-5, RUNX2, OSX, and ALP increased alone with the differentiation. Inhibition of lnc-NTF3-5 decreased the expression of RUNX2, OSX, and ALP both at mRNA and protein levels. Alizarin red staining showed similar trend. In contrast, overexpression of lnc-NTF3-5 presented totally opposite effects. Besides, overexpression of lnc-NTF3-5 could decrease the expression of microRNA-93-3p (miR-93-3p). Enhance miR-93-3p could also inhibit the expression level of lnc-NTF3-5. RNA immunoprecipitation demonstrated that lnc-NTF3-5 is directly bound to miR-93-3p and dual luciferase reporter assay proved that miR-93-3p targets 3′ UTR of RUNX2 to regulate its expression. Ultimately, in vivo bone formation study showed that lnc-NTF3-5 and miR-93-3p inhibitor co-transfection group displayed the strongest bone formation. Conclusions: The novel pathway lnc-NTF3-5/miR-93-3p/RUNX2 could regulate osteogenic differentiation of MSMSCs and might serve as a therapeutic target for bone regeneration in the posterior maxilla. © 2017 The Authors Clinical Implant Dentistry and Related Research Published by Wiley Periodicals, Inc.