Cropland-to-Miscanthus conversion alters soil bacterial and archaeal communities influencing N-cycle in Northern China
Miscanthus spp. are increasingly cultivated in cropland worldwide due to their bioenergy potential and multiple ecological services. Effects of long-term cropland-to-Miscanthus conversion without N fertilizer on soil microbiome and N cycling largely remain unknown. We aimed to explore the effects of...
Main Authors: | , , , , , , , , , , , , , |
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Format: | Article |
Language: | English |
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John Wiley and Sons Inc
2021
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Online Access: | View Fulltext in Publisher |
LEADER | 04268nam a2200745Ia 4500 | ||
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001 | 10.1111-gcbb.12874 | ||
008 | 220427s2021 CNT 000 0 und d | ||
020 | |a 17571693 (ISSN) | ||
245 | 1 | 0 | |a Cropland-to-Miscanthus conversion alters soil bacterial and archaeal communities influencing N-cycle in Northern China |
260 | 0 | |b John Wiley and Sons Inc |c 2021 | |
856 | |z View Fulltext in Publisher |u https://doi.org/10.1111/gcbb.12874 | ||
520 | 3 | |a Miscanthus spp. are increasingly cultivated in cropland worldwide due to their bioenergy potential and multiple ecological services. Effects of long-term cropland-to-Miscanthus conversion without N fertilizer on soil microbiome and N cycling largely remain unknown. We aimed to explore the effects of Miscanthus conversion on soil microbiome and N cycling over a 15-year period. We analyzed diversity, composition, and abundance of bacterial and archaeal communities using 16S rRNA amplicon sequencing, and abundances of N-cycling-related genes using quantitative polymerase chain reaction of 0–10 cm soils collected from bare land, cropland, 10-year Miscanthus × giganteus, and 15-year Miscanthus sacchriflorus land in Beijing. Conversion decreased soil sand and micro-aggregate proportion, nitrate N (NiN), available phosphorus levels, conductivity, temperature, and pH, while increasing proportion of soil clay and macro-aggregate (MAA), soil organic C (SOC), available N (AN), exchangeable Mg2+ (EMg2+), and available potassium (AK) contents as well as microbial C/N. Consequently, diversity, composition, and abundance of soil bacterial community exhibited larger changes than those values of archaeal community after conversion. Soil AP, EMg2+, AK, and SOC were key factors in shifting microbiome from the cropland to Miscanthus pattern. Moreover, abundances of bacterial and archaeal communities and the N fixer gene nifH increased, whereas that of the bacterial ammonia monooxygenase gene decreased. The copies of other N-cycling-related genes in the two Miscanthus lands seemed similar to those values of cropland. The nifH copies negatively correlated with soil NiN and positively correlated with AN, EMg2+, ECa2+, SOC, AK, and MAA. We conclude that changes in soil microbiome pattern induced by the variation of soil properties enhance microbial N fixation potential, maintaining stable N levels and robust N cycling with lower N leakage risk after conversion. These results should inspire farmers and governments to large-scale use Miscanthus on marginal cropland in Northern China. © 2021 The Authors. GCB Bioenergy published by John Wiley & Sons Ltd. | |
650 | 0 | 4 | |a 16S rRNA sequencing |
650 | 0 | 4 | |a abundance |
650 | 0 | 4 | |a Aggregates |
650 | 0 | 4 | |a agricultural land |
650 | 0 | 4 | |a Ammonia |
650 | 0 | 4 | |a Ammonia monooxygenase |
650 | 0 | 4 | |a Archaea |
650 | 0 | 4 | |a Available phosphorus |
650 | 0 | 4 | |a Available potassiums |
650 | 0 | 4 | |a Bacteria (microorganisms) |
650 | 0 | 4 | |a bacterium |
650 | 0 | 4 | |a Beijing [China] |
650 | 0 | 4 | |a Bioenergy potential |
650 | 0 | 4 | |a China |
650 | 0 | 4 | |a community dynamics |
650 | 0 | 4 | |a cropland |
650 | 0 | 4 | |a Ecological services |
650 | 0 | 4 | |a ecosystem service |
650 | 0 | 4 | |a functional genes |
650 | 0 | 4 | |a gene |
650 | 0 | 4 | |a Genes |
650 | 0 | 4 | |a Magnesium compounds |
650 | 0 | 4 | |a Microaggregates |
650 | 0 | 4 | |a Miscanthus |
650 | 0 | 4 | |a Miscanthus |
650 | 0 | 4 | |a N cycling |
650 | 0 | 4 | |a Nickel compounds |
650 | 0 | 4 | |a nitrogen cycle |
650 | 0 | 4 | |a polymerase chain reaction |
650 | 0 | 4 | |a Polymerase chain reaction |
650 | 0 | 4 | |a Quantitative polymerase chain reaction |
650 | 0 | 4 | |a RNA |
650 | 0 | 4 | |a RNA |
650 | 0 | 4 | |a Soil bacterial community |
650 | 0 | 4 | |a soil microbiome |
650 | 0 | 4 | |a soil microorganism |
650 | 0 | 4 | |a soil property |
650 | 0 | 4 | |a Soils |
700 | 1 | |a Fan, R. |e author | |
700 | 1 | |a Fan, X. |e author | |
700 | 1 | |a Guo, Q. |e author | |
700 | 1 | |a Hou, X. |e author | |
700 | 1 | |a Hou, Y. |e author | |
700 | 1 | |a Li, C. |e author | |
700 | 1 | |a Li, X. |e author | |
700 | 1 | |a Shi, R. |e author | |
700 | 1 | |a Song, J. |e author | |
700 | 1 | |a Wang, C. |e author | |
700 | 1 | |a Wu, J. |e author | |
700 | 1 | |a Yue, Y. |e author | |
700 | 1 | |a Zhang, W. |e author | |
700 | 1 | |a Zhao, C. |e author | |
773 | |t GCB Bioenergy |