A comparison of wound healing rate following treatment with Aftamed and chlorine dioxide gels in streptozotocin-induced diabetic rats

• Main Authors: Abdulla, M.A (Author), Al-Bayaty, F. (Author)
• Format: Article
• Language: English
• Subjects: aftamed; animal experiment; animal model; antioxidant activity; article; blood vessel; cell proliferation; chemical analysis; chlorine dioxide; collagen synthesis; comparative study; controlled study; enzyme activity; glutathione peroxidase; granulation tissue; histology; hyaluronic acid; hydroxyproline; lipid peroxidation; male; malonaldehyde; nonhuman; priority journal; protein analysis; rat; skin injury; streptozocin diabetes; superoxide dismutase; unclassified drug; wound closure; wound healing; wound healing promoting agent
• Online Access: View Fulltext in Publisher; View in Scopus
• ISBN: 1741427X (ISSN)
• DOI: 10.1155/2012/468764

Background and Purpose. This study aimed to evaluate the wound healing activities of Aftamed and chlorine dioxide gels in streptozotocin-induced diabetic rats. Experimental Approach. Forty-eight Sprague Dawley rats were chosen for this study, divided into 4 groups. Diabetes was induced. Two-centimeter-diameter full-thickness skin excision wounds were created. Animals were topically treated twice daily. Groups 1, the diabetic control group, were treated with 0.2mL of sterile distilled water. Group 2 served as a reference standard were treated with 0.2mL of Intrasite gel. Groups 3 and 4 were treated with 0.2mL of Aftamed and 0.2mL of chlorine dioxide gels respectively. Granulation tissue was excised on the 10th day and processed for histological and biochemical analysis. The glutathione peroxidase,superoxide dismutase activities and the malondialdehyde (MDA) levels were determined. Results. Aftamed-treated wounds exhibited significant increases in hydroxyproline, cellular proliferation, the number of blood vessels, and the level of collagen synthesis. Aftamed induced an increase in the free radical-scavenging enzyme activity and significantly reduced the lipid peroxidation levels in the wounds as measured by the reduction in the MDA level. Conclusions. This study showed that Aftamed gel is able to significantly accelerate the process of wound healing in diabetic rats. Copyright © 2012 Fouad Al-Bayaty and Mahmood Ameen Abdulla.