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03810nam a2200613Ia 4500 |
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10.1186-s12859-021-03997-w |
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220427s2021 CNT 000 0 und d |
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|a 14712105 (ISSN)
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|a geneRFinder: gene finding in distinct metagenomic data complexities
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|b BioMed Central Ltd
|c 2021
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|z View Fulltext in Publisher
|u https://doi.org/10.1186/s12859-021-03997-w
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|a Background: Microbes perform a fundamental economic, social, and environmental role in our society. Metagenomics makes it possible to investigate microbes in their natural environments (the complex communities) and their interactions. The way they act is usually estimated by looking at the functions they play in those environments and their responsibility is measured by their genes. The advances of next-generation sequencing technology have facilitated metagenomics research however it also creates a heavy computational burden. Large and complex biological datasets are available as never before. There are many gene predictors available that can aid the gene annotation process though they lack handling appropriately metagenomic data complexities. There is no standard metagenomic benchmark data for gene prediction. Thus, gene predictors may inflate their results by obfuscating low false discovery rates. Results: We introduce geneRFinder, an ML-based gene predictor able to outperform state-of-the-art gene prediction tools across this benchmark by using only one pre-trained Random Forest model. Average prediction rates of geneRFinder differed in percentage terms by 54% and 64%, respectively, against Prodigal and FragGeneScan while handling high complexity metagenomes. The specificity rate of geneRFinder had the largest distance against FragGeneScan, 79 percentage points, and 66 more than Prodigal. According to McNemar’s test, all percentual differences between predictors performances are statistically significant for all datasets with a 99% confidence interval. Conclusions: We provide geneRFinder, an approach for gene prediction in distinct metagenomic complexities, available at gitlab.com/r.lorenna/generfinder and https://osf.io/w2yd6/, and also we provide a novel, comprehensive benchmark data for gene prediction—which is based on The Critical Assessment of Metagenome Interpretation (CAMI) challenge, and contains labeled data from gene regions—available at https://sourceforge.net/p/generfinder-benchmark. © 2021, The Author(s).
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|a algorithm
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|a Algorithms
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|a article
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|a Bacteria
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|a benchmarking
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|a Benchmarking
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|a Computational burden
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|a Confidence interval
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|a Critical assessment
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|a Data handling
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|a Decision trees
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|a false discovery rate
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|a False discovery rate
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|a Forecasting
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|a Gene prediction
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|a Genes
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|a high throughput sequencing
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|a high throughput sequencing
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|a High-Throughput Nucleotide Sequencing
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|a HTTP
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|a human
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|a Large dataset
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|a Machine learning
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|a metagenome
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|a metagenome
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|a Metagenome
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|a metagenomics
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|a Metagenomics
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|a Metagenomics
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|a molecular genetics
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|a Molecular Sequence Annotation
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|a Natural environments
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|a Next-generation sequencing
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|a Percentage points
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|a prediction
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|a random forest
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|a Random forest modeling
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|a Alves, R.
|e author
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|a Góes, F.
|e author
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|a Padovani, K.
|e author
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|a Silva, R.
|e author
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|t BMC Bioinformatics
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