Paired-end small RNA sequencing reveals a possible overestimation in the isomiR sequence repertoire previously reported from conventional single read data analysis
Background: Next generation sequencing has allowed the discovery of miRNA isoforms, termed isomiRs. Some isomiRs are derived from imprecise processing of pre-miRNA precursors, leading to length variants. Additional variability is introduced by non-templated addition of bases at the ends or editing o...
Main Authors: | , , , |
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Format: | Article |
Language: | English |
Published: |
BioMed Central Ltd
2021
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Subjects: | |
Online Access: | View Fulltext in Publisher |