Paired-end small RNA sequencing reveals a possible overestimation in the isomiR sequence repertoire previously reported from conventional single read data analysis

Paired-end small RNA sequencing reveals a possible overestimation in the isomiR sequence repertoire previously reported from conventional single read data analysis

Background: Next generation sequencing has allowed the discovery of miRNA isoforms, termed isomiRs. Some isomiRs are derived from imprecise processing of pre-miRNA precursors, leading to length variants. Additional variability is introduced by non-templated addition of bases at the ends or editing o...

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Bibliographic Details
Main Authors: Luna de Haro, A. (Author), Pluvinet, R. (Author), Sanchez Herrero, J.F (Author), Sumoy, L. (Author)
Format: Article
Language:English
Published: BioMed Central Ltd 2021
Subjects:
Biological variation
data analysis
Data Analysis
Data handling
genetics
high throughput sequencing
High-Throughput Nucleotide Sequencing
IsomiR
Length variants
microRNA
MicroRNAs
miRNA
Next-generation sequencing
Paired-end sequencing
RNA
sequence analysis
Sequence Analysis, RNA
Sequencing errors
Small RNA
Technical noise
Templated
Terminal lengths
whole exome sequencing
Whole Exome Sequencing
Online Access:View Fulltext in Publisher

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