Systematic analysis of the sugar accumulation mechanism in sucrose- and hexose- accumulating cherry tomato fruits

Background: Sugar content is an important indicator of fruit quality. Except for a few wild tomato species that accumulate sucrose in the fruits, most cultivated tomato species accumulate hexose. Although several studies have focused on wild sucrose-accumulating tomato, the sucrose accumulation mech...

Full description

Bibliographic Details
Main Authors: Du, X. (Author), Li, C. (Author), Lian, L. (Author), Liu, W. (Author), Qin, Y. (Author), Song, J. (Author), Sun, L. (Author), Wang, J. (Author), Yang, L. (Author), Yang, R. (Author), Zhao, W. (Author)
Format: Article
Language:English
Published: BioMed Central Ltd 2022
Subjects:
Online Access:View Fulltext in Publisher
LEADER 02904nam a2200313Ia 4500
001 10.1186-s12870-022-03685-8
008 220630s2022 CNT 000 0 und d
020 |a 14712229 (ISSN) 
245 1 0 |a Systematic analysis of the sugar accumulation mechanism in sucrose- and hexose- accumulating cherry tomato fruits 
260 0 |b BioMed Central Ltd  |c 2022 
520 3 |a Background: Sugar content is an important indicator of fruit quality. Except for a few wild tomato species that accumulate sucrose in the fruits, most cultivated tomato species accumulate hexose. Although several studies have focused on wild sucrose-accumulating tomato, the sucrose accumulation mechanism is still unclear. Results: Here, two homozygous inbred cherry tomato lines (‘TB0023’ and ‘TB0278’, which accumulated sucrose and hexose, respectively) were selected to analyze the sugar accumulation mechanism. Carbohydrate analysis, cytological observation, gene expression and enzyme activity analysis and proteomics methods were used in this study. The results indicated that glucose and fructose were absolutely dominant in the soluble sugar content of hexose-accumulating cherry tomato fruit, while sucrose and a certain proportion of hexose were the main forms of soluble sugar in sucrose-accumulating cherry tomato fruit. The phloem unloading pathway of the hexose-accumulating cherry tomato fruit switched from symplastic to apoplastic during fruit development, and the sucrose-accumulating cherry tomato probably had a mixed unloading pathway involving the symplastic and apoplastic. High activity of acid invertase (AI), sucrose phosphate synthase (SPS), sucrose synthase (SS) and sugar transporters LeSUT1, SlSWEET2a and SlSWEET12c were important factors for hexose accumulation in the hexose-accumulating cherry tomato fruit, while LeSUT2, SPS, SS, SlSWEET1b, SlSWEET5b, SlSWEET11b, SlSWEET7a, SlSWEET14 were responsible for solute sugar accumulation in the sucrose-accumulating cherry tomato. Conclusions: This study provides detailed evidence for elucidation of the tomato sugar accumulation mechanism from the perspective of cell structure, physiology and molecular biology, providing a theoretical basis for the improvement of tomato quality and aiding the utilization of tomato genetic resources. © 2022, The Author(s). 
650 0 4 |a Accumulation 
650 0 4 |a Cherry tomato 
650 0 4 |a Hexose 
650 0 4 |a Phloem unloading 
650 0 4 |a Sucrose 
700 1 0 |a Du, X.  |e author 
700 1 0 |a Li, C.  |e author 
700 1 0 |a Lian, L.  |e author 
700 1 0 |a Liu, W.  |e author 
700 1 0 |a Qin, Y.  |e author 
700 1 0 |a Song, J.  |e author 
700 1 0 |a Sun, L.  |e author 
700 1 0 |a Wang, J.  |e author 
700 1 0 |a Yang, L.  |e author 
700 1 0 |a Yang, R.  |e author 
700 1 0 |a Zhao, W.  |e author 
773 |t BMC Plant Biology 
856 |z View Fulltext in Publisher  |u https://doi.org/10.1186/s12870-022-03685-8