Intramolecular ligation method (iLIME) for pre-miRNA quantification and sequencing
Hairpin-containing pre-miRNAs, produced from pri-miRNAs, are precursors of miRNAs (microRNAs) that play essential roles in gene expression and various human diseases. Current qPCR-based methods used to quantify pre-miRNAs are not effective to discriminate between pre-miRNAs and their parental pri-mi...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Cold Spring Harbor Laboratory Press
2022
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| Subjects: | |
| Online Access: | View Fulltext in Publisher |