Induction of apoptotic mechanism by Streblus asper root extract on cervical cancer using in vitro and in vivo models

• Main Authors: Dzulkarnain, S.M.H (Author), Ismail, W.I (Author), Nabil, M. (Author), Rahim, N.A (Author), Seeni, A. (Author)
• Format: Article
• Language: English
• Published: Oriental Scientific Publishing Company 2019
• Subjects: animal cell; animal experiment; animal model; animal tissue; Anti-cancer; antineoplastic activity; antineoplastic agent; apoptosis; Apoptosis; Article; autopsy; binding assay; cancer tissue; caspase 3; caspase 8; caspase 9; cell proliferation assay; cell viability; Cervical cancer; chaperonin 60; comparative study; controlled study; down regulation; female; HeLa cell line; IC50; livin; Moraceae; mouse; Natural plant; nonhuman; plant extract; plant root; proapoptotic activity; protein analysis; protein expression; protein p53; qualitative analysis; signal transduction; Streblus asper; Streblus asper extract; survivin; tumor invasion; tumor necrosis factor; tumor weight; Tumour Necrosis Factor; TUNEL assay; unclassified drug; upregulation; uterine cervix cancer
• Online Access: View Fulltext in Publisher; View in Scopus
• ISBN: 09746242 (ISSN)
• ISSN: 09746242 (ISSN)
• DOI: 10.13005/bpj/1796

Cervical cancer has been ranked as the third most common cancer among women worldwide. As an alternative to existing preventive and treatment measures, natural plants have been seen to carry potential therapeutic value against cancers. These include Streblus asper which proved to possess anti-cancer effects on several types of cancer. In the present study, we observed that S.asper is able to induce apoptosis on cervical cancer cells through the regulation of several apoptotic proteins. This analysis was performed using both in vitro and in vivo models. The protein expression was analysed using antibody array, 28 protein markers were found differentially expressed in both study models. Based on these findings, we propose that S.asper induces apoptosis on cervical cancer cells through TNF signaling which in turn triggers the activation of SMAC pathway and blockage of NF-?B cascade. It is also suggested that the apoptosis inducement is assisted by the HSP60 downregulation which subsequently results in p53 activation and survivin down-expression. Our study provides a preliminary understanding on selective apoptotic mechanism induced by S.asper on cervical cancer. © 2019 Oriental Scientific Publishing Company. All rights reserved.