| Summary: | Background: Induced mutagenesis has proved as a crucial implement to create genetic vari ability for various essential traits. Trachyspermum ammi is one of the most important medicinal seed spices and its oil exhibits various pharmacological properties. Ajwain has been overlooked owing its narrow genetic base and little study has been performed to create genetic variations. Methods: Dry and healthy seeds of ajwain were treated with the different concentration of gamma rays (25, 50, 75 and 100Gy), EMS (0.1%, 0.2%, 0.3% and 0.4%EMS) and different doses of combination treatments (25+0.1%, 50+0.2%, 75+0.3% and 100Gy+0.4%EMS). Among both the mutagens, gamma rays were found to be more effectual mutagens as compared to EMS. Result: The aim of the present study was to f i nd out the mutageni c consequences of gamma rays, EMS and combination treatments on growth and physi o-biochemical parameters of ajwain. Studies show that higher doses of both the mutagens caused significantly negative effect on the growth parameters whereas l ower doses have positively influenced the parameters. Result shows lower doses of mutagens (25, 50Gy, 0.1%, 0.2%EMS and 25+0.1%, 50Gy+0.2%EMS) proved to be more effective as i t caused less biological damage and theref ore would be suitable f or inducing the desirable mutat ions and improving the agronomi c trai ts in ajwain. © 2022, Agricultural Research Communication Centre. All rights reserved.
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