Modulatory activity of a bovine hydrolyzed collagen-hydroxyapatite food complex on human primary osteoblasts after simulating its gastrointestinal digestion and absorption

• Main Authors: Díez-Sánchez, E. (Author), Nieto, J.A (Author), Soriano-Romaní, L. (Author), Tomás-Cobos, L. (Author)
• Format: Article
• Language: English
• Published: ARAN Ediciones S.L 2022
• Subjects: alkaline phosphatase; Alkaline Phosphatase; animal; Animals; biological marker; Biomarkers; bone development; bovine; Caco-2 cell line; Caco-2 Cells; Cattle; cell differentiation; Cell Differentiation; collagen; Collagen; Core Binding Factor Alpha 1 Subunit; digestion; Digestion; Durapatite; genetics; human; Humans; Hydrolyzed collagen; hydroxyapatite; Hydroxyapatite; metabolism; osteoblast; Osteoblasts; osteocalcin; Osteocalcin; Osteogenesis; Osteoporosis; peptide; Peptides; transcription factor RUNX2
• Online Access: View Fulltext in Publisher

 Introduction: osteoporosis is the most prevalent bone disease and one of the main causes of chronic disability in middle and advanced ages. Conventional pharmacological treatments are still limited, and their prolonged use can cause adverse effects that motivate poor adherence to treatment. Nutritional strategies are traditionally based on supplementing the diet with calcium and vitamin D. Recent studies confirm that the results of this supplementation are significantly improved if it is accompanied by the intake of oral hydrolyzed collagen. Objective: to evaluate the possible in vitro osteogenic activity of a peptide-mineral complex formed by bovine hydrolyzed collagen and bovine hydroxyapatite (Phoscollagen®, PHC®). Methods: the digestion and absorption of PHC® were simulated using the dynamic gastrointestinal digester of AINIA and Caco-2 cell model, respectively. Primary cultures of human osteoblasts were treated with the resulting fraction of PHC® and changes were evaluated in the proliferation of preosteoblasts and in the mRNA expression of osteogenic biomarkers at different stages of osteoblast maturation: Runt-related transcription factor 2 (Runx2), alkaline phosphatase (ALP), osteocalcin (OC) and type I collagen (ColA1). Results: an increase in preosteoblastic proliferation was observed (p ≤ 0,05). No changes were detected in the biomarkers of osteoblasts with 5 days of differentiation, but with 14 days, registering in this case an increase in Runx2 (p = 0.0008), ColA1 (p = 0.035), OC (p = 0.027) and ALP (without significance). Conclusion: these results show that PHC® peptide-mineral complex stimulates the activity of mature osteoblasts, being capable of promoting bone formation. © 2022 SENPE an d Arán Ediciones S.L.