Determination of 10-gingerol in Indian ginger by validated HPTLC method of samples collected across subcontinent of India
Objective: A simple, sensitive, precise, and accurate stability indicating HPTLC (high-performance thin-layer chromatography) method for analysis of 10-gingerol in ginger has been developed and validated as per ICH guidelines. Methods: The separation was achieved on TLC (thin layer chromatography) a...
Main Authors: | , , |
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Format: | Article |
Language: | English |
Published: |
Innovare Academics Sciences Pvt. Ltd
2016
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Subjects: | |
Online Access: | View Fulltext in Publisher View in Scopus |
Summary: | Objective: A simple, sensitive, precise, and accurate stability indicating HPTLC (high-performance thin-layer chromatography) method for analysis of 10-gingerol in ginger has been developed and validated as per ICH guidelines. Methods: The separation was achieved on TLC (thin layer chromatography) aluminum plates pre-coated with silica gel 60F254 using n-hexane: ethyl acetate 55:45 (%, v/v) as a mobile phase. Densitometric analysis was performed at 569 nm. Results: This system was found to have a compact spot of 10-gingerol at RFvalue of 0.57±0.03. For the proposed procedure, linearity (r2= 0.998±0.02), limit of detection (18ng/spot), limit of quantification (42 ng/spot), recovery (ranging from 98.35%–100.68%), were found to be satisfactory. Conclusion: Statistical analysis reveals that the content of 10-gingerol in different geographical region varied significantly. The highest and lowest concentration of 10-gingerol in ginger was found to be present in a sample of Patna, Lucknow and Surat respectively which inferred that the variety of ginger found in Patna, Lucknow are much superior to other regions of India. © 2016 The Authors. |
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ISBN: | 09751491 (ISSN) |
ISSN: | 09751491 (ISSN) |
DOI: | 10.22159/ijpps.2016v8i12.14953 |