Determination of 10-gingerol in Indian ginger by validated HPTLC method of samples collected across subcontinent of India
Objective: A simple, sensitive, precise, and accurate stability indicating HPTLC (high-performance thin-layer chromatography) method for analysis of 10-gingerol in ginger has been developed and validated as per ICH guidelines. Methods: The separation was achieved on TLC (thin layer chromatography) a...
Main Authors: | , , |
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Format: | Article |
Language: | English |
Published: |
Innovare Academics Sciences Pvt. Ltd
2016
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Subjects: | |
Online Access: | View Fulltext in Publisher View in Scopus |
LEADER | 02690nam a2200421Ia 4500 | ||
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001 | 10.22159-ijpps.2016v8i12.14953 | ||
008 | 220120s2016 CNT 000 0 und d | ||
020 | |a 09751491 (ISSN) | ||
245 | 1 | 0 | |a Determination of 10-gingerol in Indian ginger by validated HPTLC method of samples collected across subcontinent of India |
260 | 0 | |b Innovare Academics Sciences Pvt. Ltd |c 2016 | |
520 | 3 | |a Objective: A simple, sensitive, precise, and accurate stability indicating HPTLC (high-performance thin-layer chromatography) method for analysis of 10-gingerol in ginger has been developed and validated as per ICH guidelines. Methods: The separation was achieved on TLC (thin layer chromatography) aluminum plates pre-coated with silica gel 60F254 using n-hexane: ethyl acetate 55:45 (%, v/v) as a mobile phase. Densitometric analysis was performed at 569 nm. Results: This system was found to have a compact spot of 10-gingerol at RFvalue of 0.57±0.03. For the proposed procedure, linearity (r2= 0.998±0.02), limit of detection (18ng/spot), limit of quantification (42 ng/spot), recovery (ranging from 98.35%–100.68%), were found to be satisfactory. Conclusion: Statistical analysis reveals that the content of 10-gingerol in different geographical region varied significantly. The highest and lowest concentration of 10-gingerol in ginger was found to be present in a sample of Patna, Lucknow and Surat respectively which inferred that the variety of ginger found in Patna, Lucknow are much superior to other regions of India. © 2016 The Authors. | |
650 | 0 | 4 | |a (10) gingerol |
650 | 0 | 4 | |a 10-gingerol |
650 | 0 | 4 | |a analytical parameters |
650 | 0 | 4 | |a Article |
650 | 0 | 4 | |a concentration (parameters) |
650 | 0 | 4 | |a geographical variation (species) |
650 | 0 | 4 | |a ginger |
650 | 0 | 4 | |a Ginger |
650 | 0 | 4 | |a high performance thin layer chromatography |
650 | 0 | 4 | |a HPTLC |
650 | 0 | 4 | |a India |
650 | 0 | 4 | |a limit of detection |
650 | 0 | 4 | |a limit of quantitation |
650 | 0 | 4 | |a linearity |
650 | 0 | 4 | |a measurement accuracy |
650 | 0 | 4 | |a nonhuman |
650 | 0 | 4 | |a phytochemistry |
650 | 0 | 4 | |a refraction index |
650 | 0 | 4 | |a robustness |
650 | 0 | 4 | |a Validation |
650 | 0 | 4 | |a validation process |
700 | 1 | 0 | |a Ali Shah, S.A. |e author |
700 | 1 | 0 | |a Ashraf, K. |e author |
700 | 1 | 0 | |a Mujeeb, M. |e author |
773 | |t International Journal of Pharmacy and Pharmaceutical Sciences |x 09751491 (ISSN) |g 8 12, 190-193 | ||
856 | |z View Fulltext in Publisher |u https://doi.org/10.22159/ijpps.2016v8i12.14953 | ||
856 | |z View in Scopus |u https://www.scopus.com/inward/record.uri?eid=2-s2.0-85003864840&doi=10.22159%2fijpps.2016v8i12.14953&partnerID=40&md5=6bc5325ab39dededc1004ec9c7e14b3f |