Determination of 10-gingerol in Indian ginger by validated HPTLC method of samples collected across subcontinent of India

• Main Authors: Ali Shah, S.A (Author), Ashraf, K. (Author), Mujeeb, M. (Author)
• Format: Article
• Language: English
• Published: Innovare Academics Sciences Pvt. Ltd 2016
• Subjects: (10) gingerol; 10-gingerol; analytical parameters; Article; concentration (parameters); geographical variation (species); ginger; Ginger; high performance thin layer chromatography; HPTLC; India; limit of detection; limit of quantitation; linearity; measurement accuracy; nonhuman; phytochemistry; refraction index; robustness; Validation; validation process
• Online Access: View Fulltext in Publisher; View in Scopus

 Objective: A simple, sensitive, precise, and accurate stability indicating HPTLC (high-performance thin-layer chromatography) method for analysis of 10-gingerol in ginger has been developed and validated as per ICH guidelines. Methods: The separation was achieved on TLC (thin layer chromatography) aluminum plates pre-coated with silica gel 60F254 using n-hexane: ethyl acetate 55:45 (%, v/v) as a mobile phase. Densitometric analysis was performed at 569 nm. Results: This system was found to have a compact spot of 10-gingerol at RFvalue of 0.57±0.03. For the proposed procedure, linearity (r2= 0.998±0.02), limit of detection (18ng/spot), limit of quantification (42 ng/spot), recovery (ranging from 98.35%–100.68%), were found to be satisfactory. Conclusion: Statistical analysis reveals that the content of 10-gingerol in different geographical region varied significantly. The highest and lowest concentration of 10-gingerol in ginger was found to be present in a sample of Patna, Lucknow and Surat respectively which inferred that the variety of ginger found in Patna, Lucknow are much superior to other regions of India. © 2016 The Authors.