Identification and characterization of a membrane receptor that binds to human STC1

Stanniocalcin-1 (STC1) is a hypocalcemic hormone originally identified in bony fishes. The mammalian homolog is found to be involved in inflammation and carcinogenesis, among other physiological functions. In this study, we used the TriCEPS-based ligand-receptor methodology to identify the putative...

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Bibliographic Details
Main Authors: Lee, W.K (Author), Ng, A.H (Author), Shi, F. (Author), Wan, H.T (Author), Wong, C.K.-C (Author)
Format: Article
Language:English
Published: NLM (Medline) 2022
Online Access:View Fulltext in Publisher
LEADER 01791nam a2200181Ia 4500
001 10.26508-lsa.202201497
008 220718s2022 CNT 000 0 und d
020 |a 25751077 (ISSN) 
245 1 0 |a Identification and characterization of a membrane receptor that binds to human STC1 
260 0 |b NLM (Medline)  |c 2022 
856 |z View Fulltext in Publisher  |u https://doi.org/10.26508/lsa.202201497 
520 3 |a Stanniocalcin-1 (STC1) is a hypocalcemic hormone originally identified in bony fishes. The mammalian homolog is found to be involved in inflammation and carcinogenesis, among other physiological functions. In this study, we used the TriCEPS-based ligand-receptor methodology to identify the putative binding proteins of human STC1 (hSTC1) in the human leukemia monocytic cell line, ThP-1. LC-MS/MS analysis of peptides from shortlisted hSTC1-binding proteins detected 32 peptides that belong to IGF2/MPRI. Surface plasmon resonance assay demonstrated that hSTC1 binds to immobilized IGF2R/MPRI with high affinity (10-20 nM) and capacity (Rmax 70-100%). The receptor binding data are comparable with those of (CREG) cellular repressor of E1A-stimulated gene a known ligand of IGF2R/MPRI, with Rmax of 75-80% and affinity values of 1-2 nM. The surface plasmon resonance competitive assays showed CREG competed with hSTC1 in binding to IGF2R/MPRI. The biological effects of hSTC1 on ThP-1 cells were demonstrated via IGF2R/MPRI to significantly reduce secreted levels of IL-1β. This is the first study to reveal the high-affinity binding of hSTC1 to the membrane receptor IGF2R/MPRI. © 2022 Wan et al. 
700 1 |a Lee, W.K.  |e author 
700 1 |a Ng, A.H.  |e author 
700 1 |a Shi, F.  |e author 
700 1 |a Wan, H.T.  |e author 
700 1 |a Wong, C.K.-C.  |e author 
773 |t Life science alliance