Spreadsheet and iteration dead times of 2-alkanones homologues series eluted by acetonitrile/water in reversed phase high-performance liquid chromatography
The dead time determination with the spreadsheet method has been conducted on the HPLC system with 2-alkanones homolog series, eluted with several composition ratios of acetonitrile/water solvent. The accuracy of the spreadsheet method is compared against the iteration method, according to the princ...
Main Authors: | , , , , , , , |
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Format: | Article |
Language: | English |
Published: |
Rasayan Journal of Chemistry, c/o Dr. Pratima Sharma
2021
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Subjects: | |
Online Access: | View Fulltext in Publisher |
Summary: | The dead time determination with the spreadsheet method has been conducted on the HPLC system with 2-alkanones homolog series, eluted with several composition ratios of acetonitrile/water solvent. The accuracy of the spreadsheet method is compared against the iteration method, according to the principle of (1) the smallest dead time; (2) the smallest standard deviation of the different dead time values given by the different solvent composition ratios; and (3) the highest linearity of the correlation between the natural logarithm of capacity factor ln(k´) versus carbon number nc. The comparison results suggest that the spreadsheet method has equally good criteria with the iteration method. The use of acetonitrile/water solvent exhibits the improved accuracy of the dead time determination with smaller dead time values and standard deviation than the ones given by the methanol/water solvent. © 2021, Rasayan Journal of Chemistry, c/o Dr. Pratima Sharma. All rights reserved. |
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ISBN: | 09741496 (ISSN) |
DOI: | 10.31788/RJC.2021.1426024 |