Spreadsheet and iteration dead times of 2-alkanones homologues series eluted by acetonitrile/water in reversed phase high-performance liquid chromatography

• Main Authors: Idroes, G.M (Author), Idroes, R. (Author), Irvanizam, I. (Author), Mahmudi, M. (Author), Muslem, M. (Author), Rusyana, A. (Author), Saiful, S. (Author), Suhendra, R. (Author)
• Format: Article
• Language: English
• Published: Rasayan Journal of Chemistry, c/o Dr. Pratima Sharma 2021
• Subjects: Alkanones; Dead time; Liquid chromatography; Mathematical methods; Solvent composition
• Online Access: View Fulltext in Publisher
• ISBN: 09741496 (ISSN)
• DOI: 10.31788/RJC.2021.1426024

The dead time determination with the spreadsheet method has been conducted on the HPLC system with 2-alkanones homolog series, eluted with several composition ratios of acetonitrile/water solvent. The accuracy of the spreadsheet method is compared against the iteration method, according to the principle of (1) the smallest dead time; (2) the smallest standard deviation of the different dead time values given by the different solvent composition ratios; and (3) the highest linearity of the correlation between the natural logarithm of capacity factor ln(k´) versus carbon number nc. The comparison results suggest that the spreadsheet method has equally good criteria with the iteration method. The use of acetonitrile/water solvent exhibits the improved accuracy of the dead time determination with smaller dead time values and standard deviation than the ones given by the methanol/water solvent. © 2021, Rasayan Journal of Chemistry, c/o Dr. Pratima Sharma. All rights reserved.