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|a To address the problem of antibiotic resistance in pathogens, our research aimed for endophytic actinobacteria, producers of a diverse array of significant bioactive metabolites. Endophytic actinobacteria SIR5 was isolated from roots of Sphaeranthus indicus Linn. and was identified to be Actinoalloteichus cyanogriseus via 16S rRNA sequencing. With the accession number MK793584, the gene sequence was deposited to NCBI. In the current study, a rare actinobacteria Actinoalloteichus cyanogriseus, has been reported as an endophyte for the first time. Both Microbial Type Culture Collection (MTCC) and Clinical Cultures (CC) were used to investigate the antimicrobial property of the bioactive chemicals synthesized by A. cyanogriseus SIR5. A significant zone of inhibitions was recorded against clinical cultures: B. cereus (12.16±0.16 mm), Candida albicans (12.83±0.44 mm), E. coli (15.33±0.33 mm), S. epidermidis (11.50±0.28 mm) and MTCC pathogens: B. cereus (11.16±0.16 mm), B. subtilis (13.33±0.16 mm), P. aeruginosa (13.33±0.33 mm), S. epidermidis (12.33±0.33 mm). The production of bioactive compound was enhanced by optimization using one factor at a time (OFAT), which was achieved with modified ISP-4 medium (starch-1% w/v, NH4NO3-1% w/v, CaCO3-2 g/l, K2HPO4-1 g/l, MgSO4-1 g/l, NaCl-1g/l, trace solution-1 ml/l) with inoculum size-13%, incubation period-16 days, pH-8.0 and temperature-28oC. © 2021, Rasayan Journal of Chemistry, c/o Dr. Pratima Sharma. All rights reserved.
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