Study on the detection of anthrax by ICP-MS based on gold nanoparticle labeling
BACKGROUND: In recent years, inductively coupled plasma mass spectrometry (ICP-MS) has been widely used in the field of molecular biology because of its unique advantages. Anthrax is a widespread and long-standing infectious disease, which affects and restricts people's work and life seriously....
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
NLM (Medline)
2023
|
| Subjects: | |
| Online Access: | View Fulltext in Publisher |
| LEADER | 02317nam a2200445Ia 4500 | ||
|---|---|---|---|
| 001 | 10.3233-THC-236024 | ||
| 008 | 230526s2023 CNT 000 0 und d | ||
| 020 | |a 18787401 (ISSN) | ||
| 245 | 1 | 0 | |a Study on the detection of anthrax by ICP-MS based on gold nanoparticle labeling |
| 260 | 0 | |b NLM (Medline) |c 2023 | |
| 300 | |a 10 | ||
| 856 | |z View Fulltext in Publisher |u https://doi.org/10.3233/THC-236024 | ||
| 520 | 3 | |a BACKGROUND: In recent years, inductively coupled plasma mass spectrometry (ICP-MS) has been widely used in the field of molecular biology because of its unique advantages. Anthrax is a widespread and long-standing infectious disease, which affects and restricts people's work and life seriously. OBJECTIVE: The study goal is to develop a new method for the detection of anthrax. METHODS: A rapid, sensitive and accurate method for the detection of anthrax characteristic DNA was proposed by combing gold nanoparticles (AuNPs) and inductively coupled plasma mass spectrometry. RESULTS: The linear range of this method is 100-2500 pmol/L and the limit of detection of 16.61 pmol/L. CONCLUSION: The proposed method has numerous advantages, including simplicity of operation, high sensitivity, and specificity, which provides a new idea for the detection of anthrax. Importantly, this methodology has good potential for the detection of other biological substances such as bacteria and viruses by changing the modification sequence on the nanoparticle probe. | |
| 650 | 0 | 4 | |a anthrax |
| 650 | 0 | 4 | |a Anthrax |
| 650 | 0 | 4 | |a AuNPs |
| 650 | 0 | 4 | |a chemistry |
| 650 | 0 | 4 | |a DNA |
| 650 | 0 | 4 | |a genetics |
| 650 | 0 | 4 | |a gold |
| 650 | 0 | 4 | |a Gold |
| 650 | 0 | 4 | |a human |
| 650 | 0 | 4 | |a Humans |
| 650 | 0 | 4 | |a ICP-MS |
| 650 | 0 | 4 | |a label |
| 650 | 0 | 4 | |a mass spectrometry |
| 650 | 0 | 4 | |a Mass Spectrometry |
| 650 | 0 | 4 | |a metal nanoparticle |
| 650 | 0 | 4 | |a Metal Nanoparticles |
| 650 | 0 | 4 | |a procedures |
| 700 | 1 | 0 | |a Chen, G. |e author |
| 700 | 1 | 0 | |a Chen, K. |e author |
| 700 | 1 | 0 | |a Han, L. |e author |
| 700 | 1 | 0 | |a Han, X. |e author |
| 700 | 1 | 0 | |a Liu, G. |e author |
| 700 | 1 | 0 | |a Luo, T. |e author |
| 700 | 1 | 0 | |a Ma, T. |e author |
| 700 | 1 | 0 | |a Xu, Z. |e author |
| 700 | 1 | 0 | |a Yu, M. |e author |
| 773 | |t Technology and health care : official journal of the European Society for Engineering and Medicine |x 18787401 (ISSN) |g 31 S1, 283-292 | ||