C/N‐Dependent Element Bioconversion Efficiency and Antimicrobial Protein Expression in Food Waste Treatment by Black Soldier Fly Larvae
The black soldier fly (BSF), Hermetia illucens, has emerged as a promising species for waste bioconversion and source of antimicrobial proteins (AMPs). However, there is a scarcity of research on the element transformation efficiency and molecular characterization of AMPs derived from waste manageme...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI
2022
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| Subjects: | |
| Online Access: | View Fulltext in Publisher |
| LEADER | 02589nam a2200349Ia 4500 | ||
|---|---|---|---|
| 001 | 10.3390-ijms23095036 | ||
| 008 | 220706s2022 CNT 000 0 und d | ||
| 020 | |a 16616596 (ISSN) | ||
| 245 | 1 | 0 | |a C/N‐Dependent Element Bioconversion Efficiency and Antimicrobial Protein Expression in Food Waste Treatment by Black Soldier Fly Larvae |
| 260 | 0 | |b MDPI |c 2022 | |
| 856 | |z View Fulltext in Publisher |u https://doi.org/10.3390/ijms23095036 | ||
| 520 | 3 | |a The black soldier fly (BSF), Hermetia illucens, has emerged as a promising species for waste bioconversion and source of antimicrobial proteins (AMPs). However, there is a scarcity of research on the element transformation efficiency and molecular characterization of AMPs derived from waste management. Here, food waste treatment was performed using BSF larvae (BSFL) in a C/N ratio of 21:1–10:1, with a focus on the C/N‐dependent element bioconversion, AMP antimicrobial activity, and transcriptome profiling. The C‐larvae transformation rates were found to be similar among C/Ns (27.0–35.5%, p = 0.109), while the N‐larvae rates were different (p = 0.001), with C/N 21:1–16:1 (63.5–75.0%) being higher than C/N 14:1–10:1 (35.0–45.7%). The C/N ratio did not alter the antimicrobial spectrum of AMPs, but did affect the activities, with C/N 21:1 being significantly lower than C/N 18:1–10:1. The lysozyme genes were found to be significantly more highly expressed than the cecropin, defensin, and attacin genes in the AMP gene family. Out of 51 lysozyme genes, C/N 18:1 and C/N 16:1 up‐regulated (p < 0.05) 14 and 12 genes compared with C/N 21:1, respectively, corresponding to the higher activity of AMPs. Overall, the element bioconversion efficiency and AMP expression can be enhanced through C/N ratio manipulation, and the C/N‐dependent transcriptome regulation is the driving force of the AMP difference. © 2022 by the authors. Licensee MDPI, Basel, Switzerland. | |
| 650 | 0 | 4 | |a antimicrobial proteins |
| 650 | 0 | 4 | |a black soldier fly larvae |
| 650 | 0 | 4 | |a C/N ratio |
| 650 | 0 | 4 | |a element recycle |
| 650 | 0 | 4 | |a food waste |
| 650 | 0 | 4 | |a gene family |
| 650 | 0 | 4 | |a Hermetia illucens |
| 650 | 0 | 4 | |a transcriptome |
| 700 | 1 | 0 | |a Dong, X. |e author |
| 700 | 1 | 0 | |a Guan, S. |e author |
| 700 | 1 | 0 | |a Jin, N. |e author |
| 700 | 1 | 0 | |a Liu, Y. |e author |
| 700 | 1 | 0 | |a Sun, S. |e author |
| 700 | 1 | 0 | |a Tong, H. |e author |
| 700 | 1 | 0 | |a Wu, M. |e author |
| 700 | 1 | 0 | |a Xu, J. |e author |
| 700 | 1 | 0 | |a Xu, W. |e author |
| 700 | 1 | 0 | |a Zhang, S. |e author |
| 700 | 1 | 0 | |a Zhou, H. |e author |
| 773 | |t International Journal of Molecular Sciences | ||