Transcriptomic and Metabolomic Analyses of the Effects of Exogenous Trehalose on Heat Tolerance in Wheat

Trehalose can improve the tolerance of plants to various types of environmental stress. Nonetheless, information respecting the molecular networks of wheat seedlings to exogenous tre-halose under heat stress is limited. Here, two wheat varieties pretreated with exogenous trehalose were selected to e...

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Bibliographic Details
Main Authors: Gao, Y. (Author), Lang, S. (Author), Li, W. (Author), Luo, Y. (Author), Wang, Y. (Author), Xie, Y. (Author)
Format: Article
Language:English
Published: MDPI 2022
Subjects:
Online Access:View Fulltext in Publisher
LEADER 02634nam a2200253Ia 4500
001 10.3390-ijms23095194
008 220706s2022 CNT 000 0 und d
020 |a 16616596 (ISSN) 
245 1 0 |a Transcriptomic and Metabolomic Analyses of the Effects of Exogenous Trehalose on Heat Tolerance in Wheat 
260 0 |b MDPI  |c 2022 
856 |z View Fulltext in Publisher  |u https://doi.org/10.3390/ijms23095194 
520 3 |a Trehalose can improve the tolerance of plants to various types of environmental stress. Nonetheless, information respecting the molecular networks of wheat seedlings to exogenous tre-halose under heat stress is limited. Here, two wheat varieties pretreated with exogenous trehalose were selected to explore the molecular mechanism by which trehalose improves the heat tolerance of wheat (Triticum aestivum L.). The results indicated that exogenous trehalose improved the physiological state of wheat seedlings under heat stress. Through RNA sequencing and metabolomics analysis, the genes and metabolites specifically expressed in trehalose pretreatment were identi-fied. After heat stress, there were 18,352 differentially expressed genes (DEGs) in the control and trehalose-treated (H_vs_TreH) groups of Yangmai 18 and 9045 DEGs in Yannong 19. Functional annotation and enrichment analyses showed that the DEGs in the two wheat varieties were mainly involved in carbohydrate metabolism and biosynthesis of secondary metabolites. Through a liquid chromatography–mass spectrometry platform, 183 differential metabolites in H_vs_TreH groups of Yangmai 18 and 77 differential metabolites in Yannong 19 were identified. Compared with the control group, many protective metabolites, such as amino acids, purines, phenylpropanoids and flavonoids, showed significant differences under heat stress. The results indicated that exogenous trehalose protected the wheat biomembrane system, enhanced carbohydrate metabolism and signal transduction, strengthened the activity of the tricarboxylic acid cycle (TCA cycle), regulated purine metabolism, gene expression and metabolite accumulation in the phenylpropanoid biosynthesis and flavonoid biosynthesis pathways, thus improving the heat tolerance of wheat. © 2022 by the authors. Licensee MDPI, Basel, Switzerland. 
650 0 4 |a heat stress 
650 0 4 |a metabolome 
650 0 4 |a transcriptome 
650 0 4 |a trehalose 
650 0 4 |a wheat 
700 1 0 |a Gao, Y.  |e author 
700 1 0 |a Lang, S.  |e author 
700 1 0 |a Li, W.  |e author 
700 1 0 |a Luo, Y.  |e author 
700 1 0 |a Wang, Y.  |e author 
700 1 0 |a Xie, Y.  |e author 
773 |t International Journal of Molecular Sciences