The β-1,3-Glucanase Degrades Callose at Plasmodesmata to Facilitate the Transport of the Ribonucleoprotein Complex in Pyrus betulaefolia

• Main Authors: Huang, W. (Author), Li, T. (Author), Mao, C. (Author), Tian, B. (Author), Wang, S. (Author), Xiang, L. (Author), Xu, C. (Author), Yu, Y. (Author), Zhang, X. (Author)
• Format: Article
• Language: English
• Published: MDPI 2023
• Subjects: callose deposition; long-distance movement; PbANK; Pyrus betulaefolia; ribonucleoprotein complex; β-1,3-glucanase
• Online Access: View Fulltext in Publisher; View in Scopus
• ISBN: 16616596 (ISSN)
• DOI: 10.3390/ijms24098051

Grafting is widely used to improve the stress tolerance and the fruit yield of horticultural crops. Ribonucleoprotein complexes formed by mRNAs and proteins play critical roles in the communication between scions and stocks of grafted plants. In Pyrus betulaefolia, ankyrin was identified previously to promote the long-distance movement of the ribonucleoprotein complex(PbWoxT1-PbPTB3) by facilitating callose degradation at plasmodesmata. However, the mechanism of the ankyrin-mediated callose degradation remains elusive. In this study, we discovered a β-1,3-glucanase (EC 3.2.1.39, PbPDBG) using ankyrin as a bait from plasmodesmata by co-immunoprecipitation and mass spectrometry. Ankyrin was required for the plasmodesmata-localization of PbPDBG. The grafting and bombardment experiments indicated that overexpressing PbPDBG resulted in decreased callose content at plasmodesmata, and thereby promoting the long-distance transport of the ribonucleoprotein complex. Altogether, our findings revealed that PbPDBG was the key factor in ankyrin-mediated callose degradation at plasmodesmata. © 2023 by the authors.