New Organic Salt from Levofloxacin-Citric Acid: What Is the Impact on the Stability and Antibiotic Potency?

• Main Authors: Laksana, A.N (Author), Nugrahani, I. (Author), Oyama, H. (Author), Uekusa, H. (Author)
• Format: Article
• Language: English
• Published: MDPI 2022
• Subjects: Anti-Bacterial Agents; antibiotic potency; antiinfective agent; antioxidant; Antioxidants; chemistry; citric acid; Citric Acid; infrared spectroscopy; levofloxacin; Levofloxacin; levofloxacin-citrate; organic salt; Spectroscopy, Fourier Transform Infrared; stability
• Online Access: View Fulltext in Publisher

 This research dealt with the composition, structure determination, stability, and antibiotic potency of a novel organic salt composed of levofloxacin (LF) and citric acid (CA), named levofloxacin-citrate (LC). After a stoichiometric proportion screening, the antibiotic-antioxidant reaction was conducted by slow and fast evaporation methods. A series of characterizations using thermal analysis, powder X-ray diffractometry, vibrational spectroscopy, and nuclear magnetic resonance confirmed LC formation. The new organic salt showed a distinct thermogram and diffractogram. Next, Fourier transform infrared indicated the change in N-methylamine and carboxylic stretching, confirmed by1H nuclear magnetic resonance spectra to elucidate the 2D structure. Finally, single-crystal diffractometry determined LC as a new salt structure three-dimensionally. The attributive improvements were demonstrated on the stability toward the humidity and lighting of LC compared to LF alone. Moreover, the antibiotic potency of LF against Staphylococcus aureus (Gram-positive) and Escherichia coli (Gram-negative) enhanced ~1.5–2-fold by LC. Hereafter, LC is a potential salt antibiotic-antioxidant combination for dosage formulas development. © 2022 by the authors. Licensee MDPI, Basel, Switzerland.