Peptide cross-linked poly (Ethylene glycol) hydrogel films as biosensor coatings for the detection of collagenase

• Main Authors: Ahmad, N. (Author), Colak, B. (Author), Gautrot, J.E (Author), Gibbs, M.J (Author), Krause, S. (Author), Remzi Becer, C. (Author), Watkinson, M. (Author), Zhang, D.-W (Author)
• Format: Article
• Language: English
• Published: MDPI AG 2019
• Subjects: Biosensing Techniques; Biosensors; chemistry; Click chemistry; collagenase; Collagenase; Collagenases; cross linking reagent; Crosslinking; Cross-Linking Reagents; Degradable hydrogel; Diseases; Drug delivery; Ethylene glycol; genetic procedures; Gold coatings; Gold deposits; Hydrogel degradation; Hydrogel, Polyethylene Glycol Dimethacrylate; Hydrogels; isolation and purification; limit of detection; Limit of Detection; Lower detection limit; macrogol; Norbornenes; peptide; Peptide cross-links; Peptides; Poly(ethylene glycol) hydrogel; Poly(ethylene glycol) norbornene; polyethylene glycol dimethacrylate hydrogel; Polyethylene glycols; Polyethylene Glycols; Polyols; Protease biosensor; QCM; Quartz; quartz crystal microbalance; Quartz Crystal Microbalance Techniques; Quartz crystal microbalances; Rheumatoid arthritis; Tissue engineering
• Online Access: View Fulltext in Publisher; View in Scopus
• ISBN: 14248220 (ISSN)
• ISSN: 14248220 (ISSN)
• DOI: 10.3390/s19071677

Peptide cross-linked poly(ethylene glycol) hydrogel has been widely used for drug delivery and tissue engineering. However, the use of this material as a biosensor for the detection of collagenase has not been explored. Proteases play a key role in the pathology of diseases such as rheumatoid arthritis and osteoarthritis. The detection of this class of enzyme using the degradable hydrogel film format is promising as a point-of-care device for disease monitoring. In this study, a protease biosensor was developed based on the degradation of a peptide cross-linked poly(ethylene glycol) hydrogel film and demonstrated for the detection of collagenase. The hydrogel was deposited on gold-coated quartz crystals, and their degradation in the presence of collagenase was monitored using a quartz crystal microbalance (QCM). The biosensor was shown to respond to concentrations between 2 and 2000 nM in less than 10 min with a lower detection limit of 2 nM. © 2019 by the authors. Licensee MDPI, Basel, Switzerland.