Field exposure to 50 Hz significantly affects wild type and unfolded p53 expression in NB69 neuroblastoma cells

• Main Authors: Ángeles Trillo, M. (Author), Martínez, M.A (Author), MartínezBotas, J. (Author), Úbeda, A. (Author)
• Format: Article
• Language: English
• Published: Spandidos Publications 2022
• Subjects: Bcl2; extremely low frequency; magnetic fields; NB69; p53
• Online Access: View Fulltext in Publisher

 Previous studies have shown that intermittent exposure to a 50 Hz, 100 µT sinusoidal magnetic field (MF) promotes proliferation of human neuroblastoma cells, NB69. This effect is mediated by activation of the epidermal growth factor receptor through a free radicaldependent activa tion of the p38 pathway. The present study investigated the possibility that the oxidative stresssensitive protein p53 is a potential target of the MF, and that field exposure can affect the protein expression. To that end, NB69 cells were exposed to short intervals of 30 to 120 min to the aforementioned MF parameters. Two specific antip53 antibodies that allow discrimination between the wild and unfolded forms of p53 were used to study the expression and cellular distribution of both isoforms of the protein. The expression of the antiapop totic protein Bcl2, whose regulation is mediated by p53, was also analyzed. The obtained results revealed that MF expo sure induced increases in p53 gene expression and in protein expression of the wildtype form of p53. Field exposure also caused overexpression of the unfolded form of p53, together with changes in the nuclear/cytoplasmic distribution of both forms of the protein. The expression of protein Bcl2 was also significantly increased in response to the MF. As a whole, these results indicated that the MF is capable of interacting with the function, distribution and conformation of protein p53. Such interactions could be involved in previously reported MF effects on NB69 proliferation promotion. © 2022 Spandidos Publications. All rights reserved.