Canonical Wnt pathway gene expression and their clinical correlation in oral squamous cell carcinoma

• Main Authors: Andiappan, M. (Author), Balakrishnan, A. (Author), Marimuthu, M. (Author), Muthusekhar, M. (Author), Shanmugam, S. (Author), Wahab, A. (Author)
• Format: Article
• Language: English
• Published: Medknow Publications 2018
• Subjects: Adaptor Proteins, Signal Transducing; Canonical Wnt pathway; Carcinoma, Squamous Cell; CCND1 protein, human; cyclin D1; Cyclin D1; DKK1 protein, human; DNA binding protein; DNA-Binding Proteins; eye protein; Eye Proteins; female; Female; gene analysis; gene expression regulation; Gene Expression Regulation, Neoplastic; genetics; human; Humans; Intercellular Signaling Peptides and Proteins; Intracellular Signaling Peptides and Proteins; male; Male; membrane protein; Membrane Proteins; middle aged; Middle Aged; Mouth Neoplasms; mouth tumor; MYCBP protein, human; oncoprotein; oral squamous cell carcinoma; prognosis; Prognosis; Prospective Studies; prospective study; Proto-Oncogene Proteins; quantitative reverse transcription-polymerase chain reaction; repressor protein; Repressor Proteins; Reverse Transcriptase Polymerase Chain Reaction; reverse transcription polymerase chain reaction; SFRP1 protein, human; SFRP2 protein, human; SFRP4 protein, human; SFRP5 protein, human; signal peptide; signal transducing adaptor protein; squamous cell carcinoma; transcription factor; Transcription Factors; WD repeat containing planar cell polarity effector; WIF1 protein, human; Wnt signaling; Wnt Signaling Pathway
• Online Access: View Fulltext in Publisher

 Aim: The aim of this study is to explore the prognostic significance and clinicopathological correlations of the Wnt pathway genes in a cohort of surgically treated patients with oral squamous cell carcinoma (OSCC) patients. Settings and Design: A prospective genetic study on patients with OSCC was carried out during the period from July 2014 to January 2016. Informed consent from patients and institutional ethical approval for the study was obtained and the guidelines were strictly followed for collection of samples. Subjects and Methods: Clinical data and mRNA expression analysis of ten genes in the canonical Wnt pathway were evaluated and their relationships with clinical and demographic variables were studied in 58 tissue samples. Wnt-3a, β-catenin, secreted frizzled-related proteins sFRP-1, sFRP-2, sFRP-4, sFRP-5, Wnt inhibitory factor 1, dickkopf-1, c-MYC, and cyclin-D1 from cancer (n = 29) and normal (n = 29) tissue samples were investigated using quantitative reverse transcription-polymerase chain reaction. Statistical Analysis: Descriptive statistics were used to summarize the sample characteristics and clinical variables. If the data were normal, then parametric tests were used; otherwise, nonparametric alternatives were used. All the analyses were carried out using SPSS version 23.0 (IBM SPSS Inc., USA). Results: Expression of sFRP-1, sFRP-2, and sFRP-5 in control samples and expression of c-MYC and cyclin D1 in cancer samples showed statistical significance. Significant expression of Wnt3A was observed among patients who had recurrence and were deceased. Conclusion: Wnt3A, β-catenin, and cyclin D1 are recognized as key components of Wnt/β-catenin signaling. However, in this study, there was no significant expression of all the three genes in OSCC. The proto-oncogene c-MYC showed statistically significant upregulation in cancer tissue samples suggesting that the OSCC among South Indian population is primarily not mediated by the canonical Wnt signaling pathway. © 2018 Indian Journal of Dental Research | Published by Wolters Kluwer - Medknow.