The analysis of matrix metalloproteinase-8 in gingival crevicular fluid and periodontal diseases

• Main Authors: Darwin, E. (Author), Kasuma, N. (Author), Oenzil, F. (Author), Sofyan, Y. (Author)
• Format: Article
• Language: English
• Published: Wolters Kluwer Medknow Publications 2018
• Subjects: adolescent; Adolescent; adult; Adult; biological marker; Biomarkers; Cross-Sectional Studies; cross-sectional study; enzymology; female; Female; gingival crevicular fluid; Gingival Crevicular Fluid; gingivitis; human; Humans; male; Male; Matrix Metalloproteinase 8; Matrix metalloproteinase-8; neutrophil collagenase; periodontal disease; Periodontal Diseases; young adult; Young Adult
• Online Access: View Fulltext in Publisher

 Background: Periodontal disease, also generally called periodontitis or gum disease, is a chronic infection-induced inflammatory disease that causes tooth loss if not properly treated, and is considered as a modifying factor in systemic health. Gingival crevicular fluid (GCF) matrix metalloproteinase-8 (MMP-8) is an inflammatory marker found in periodontal pathologic conditions. Gingivitis, a nondestructive type of periodontal disease, can progress to periodontitis if left untreated. Therefore, assessing the level of MMP-8 with comfortable methods and no tissue intervention can determine the progression of the periodontal disease for a better treatment. Objective: The purpose of the present study is to determine the relationship between MMP-8 in GCF and periodontal disease. Setting and Design: This is a cross-sectional study that took place in West Sumatra, Indonesia from June to December 2013. Materials and Methods: This study involves 60 respondents who are divided into three groups based on the periodontal disease index. The samples consist of 20 healthy individuals, 20 with mild gingivitis, and 20 periodontitis initial. GCF was collected from each group. MMP8 level in GCF was tested by using ELISA technique. Statistical Analysis: Data were analyzed with SPSS version 17 Software. ANOVA test was used to determine the differences in average levels of MMP-8. Bonferroni post hoc test was used to discover which spesific means differed. Results: The level of MMP-8 is significantly different between the healthy group and mild gingivitis group, between the healthy group with mild periodontitis group, and also between groups with mild gingivitis and mild periodontitis (P < 0.05). Conclusion: The findings of this study can be used by practitioners of dentistry to establish a proper diagnosis and appropriate treatment of periodontal disease by measuring the scale of MMP-8, to prevent or to minimize further complication in periodontitis patients. © 2018 Indian Journal of Dental Research.