Gauging the flexibility of fluorescent markers for the interpretation of fluorescence resonance energy transfer
Intramolecular distances in proteins and other biomolecules can be studied in living cells by means of fluorescence resonance energy transfer (FRET) in steady-state or pulsed-excitation experiments. The major uncertainty originates from the unknown orientation between the optical dipole moments of t...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
2011.
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| Subjects: | |
| Online Access: | Get fulltext |