|
|
|
|
LEADER |
01543 am a22001813u 4500 |
001 |
362872 |
042 |
|
|
|a dc
|
100 |
1 |
0 |
|a Riley, Jo-Anne
|e author
|
700 |
1 |
0 |
|a Brown, Tom
|e author
|
700 |
1 |
0 |
|a Gale, Nittaya
|e author
|
700 |
1 |
0 |
|a Herniman, Julie
|e author
|
700 |
1 |
0 |
|a Langley, G. John
|e author
|
245 |
0 |
0 |
|a Self-reporting hybridisation assay for miRNA analysis
|
260 |
|
|
|c 2014.
|
856 |
|
|
|z Get fulltext
|u https://eprints.soton.ac.uk/362872/1/Riley.pdf
|
520 |
|
|
|a Hybridisation assays, which are commonly used to analyse oligonucleotides such as siRNAs and miRNAs, often employ detection probes with fluorescent tags. The signal emitted by a fluorescent tag covers a broad range of wavelengths and this limits the multiplexing potential due to overlapping signals. A novel method of indirect oligonucleotide analysis has been developed which combines a hybridisation assay with cleavable small molecule mass tags using HPLC-ESI MS detection. A self-reporting detection probe has been designed which incorporates a DNA/RNA chimeric oligonucleotide sequence in the reporter region, which generates small nucleotide products upon RNase cleavage of the ribose-phosphate backbone. These small nucleotides can then serve as mass tags for the indirect detection of oligonucleotide analytes. The narrow mass range covered by a small molecule mass tag combined with the wide range of possible mass tags provides a high degree of multiplexing potential. This approach has been demonstrated for the analysis of a synthetic miRNA.
|
540 |
|
|
|a other
|
655 |
7 |
|
|a Article
|