Variable leucine-rich repeats of tomato disease resistance genes Cf-2 and Cf-5 determine specificity
The tomato Cf-2 and Cf-5 genes confer race specific resistance to infection by the leaf mould pathogen Cladosporium fulvum. The encoded proteins induce a defence response upon recognition of the fungal Avr2 and Avr5 determinants, respectively. Each resistance protein is comprised largely of leucine-...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
2003.
|
| Subjects: | |
| Online Access: | Get fulltext |
| LEADER | 01312 am a22001333u 4500 | ||
|---|---|---|---|
| 001 | 40511 | ||
| 042 | |a dc | ||
| 100 | 1 | 0 | |a Seear, Paul J. |e author |
| 700 | 1 | 0 | |a Dixon, Mark S. |e author |
| 245 | 0 | 0 | |a Variable leucine-rich repeats of tomato disease resistance genes Cf-2 and Cf-5 determine specificity |
| 260 | |c 2003. | ||
| 856 | |z Get fulltext |u https://eprints.soton.ac.uk/40511/1/Seear_and_Dixon_2003.pdf | ||
| 520 | |a The tomato Cf-2 and Cf-5 genes confer race specific resistance to infection by the leaf mould pathogen Cladosporium fulvum. The encoded proteins induce a defence response upon recognition of the fungal Avr2 and Avr5 determinants, respectively. Each resistance protein is comprised largely of leucine-rich repeats (LRRs) and the specificity of recognition is thought to occur through a particular domain. We have investigated this further using domain swaps between Cf-2 and Cf-5. Engineered chimeric genes containing portions of Cf-2 and Cf-5 were expressed and shown to be functional. The results clearly show that the specificity for the particular avirulence determinant is restricted to a region of each gene that encodes a subset of LRRs containing the highest level of intergenic variability. In addition, two non-functional mutants of Cf-5 were characterized and their significance discussed. | ||
| 655 | 7 | |a Article | |