Bioactive 2-(Methyldithio)Pyridine-3-Carbonitrile from Persian Shallot (Allium stipitatum Regel.) Exerts Broad-Spectrum Antimicrobial Activity

• Main Authors: Chigurupati, S (Author), Fauzi, FM (Author), Ghaznavi-Rad, E (Author), Hamat, RA (Author), Joseph, N (Author), Karunanidhi, A (Author), Lung, LTT (Author), Nathan, JJ (Author), Neela, V (Author), Pichika, MR (Author), van Belkum, A (Author)
• Format: Article
• Language: English
• Published: 2019
• Subjects: Allium stipitatum; antibacterial; COSTS; DERIVATIVES; EPIDEMIOLOGY; METHICILLIN-RESISTANT; methicillin-resistant Staphylococcus aureus; minimum inhibitory concentration; MORTALITY; NATURAL-PRODUCTS; OUTCOMES
• Online Access: View Fulltext in Publisher

 Antibiotic resistance is a problem that continues to challenge the healthcare sector, especially in clinically significant pathogens like methicillin-resistant Staphylococcus aureus (MRSA). Herein is described the isolation and structure elucidation of a bioactive compound from Allium stipitatum with antimicrobial activity. Crude Allium stipitatum dichloromethane extract (ASDE) was subjected to systematic purification by chromatographic procedures to afford various bioactive fractions. A fraction that exhibited anti-MRSA activity (4 mu gmL(-1)) was further characterized to determine the structure. The structure of the compound was elucidated as 2-(methyldithio)pyridine-3-carbonitrile (2-Medpy-3-CN). The 2-Medpy-3-CN compound, which was screened for antimicrobial activity, exhibited minimum inhibitory concentrations (MICs) in the range of 0.5 to >64 mu gmL(-1) for tested bacterial species and 0.25 to 2 mu gmL(-1) for Candida spp. Further studies are important to confirm the drug target and mechanism of action.