Article 2 CRISPR 2 CRISPR Cas system 2 CRISPR-Cas Systems 2 Cas9 Clustered Regularly Interspaced Short Palindromic Repeats 2 Gene Editing 2 clustered regularly interspaced short palindromic repeat 2 gene editing 2 gene knockout 2 genetics 2 genome editing 2 guide RNA 2 CRISPR associated endonuclease Cas9 1 CRISPR-CAS9 system 1 CRISPRi/a 1 Genome 1 Golden Gate assembly 1 Plant Breeding 1 RNA sequence 1 RNA targeting 1 RNA, Guide 1 Sanger sequencing 1 Triticum 1 Triticum aestivum 1 Triticum turgidum 1 amplicon 1 clinical protocol 1 conserved sequence 1 controlled study 1