Article 1 CRISPR 1 CRISPR Cas system 1 CRISPR associated endonuclease Cas9 1 CRISPR-CAS9 system 1 CRISPR-Cas Systems 1 Cas9 1 Clustered Regularly Interspaced Short Palindromic Repeats 1 Gene Editing 1 Golden Gate assembly Plant Breeding 1 Sanger sequencing 1 Triticum 1 Triticum aestivum 1 Triticum turgidum 1 amplicon 1 clustered regularly interspaced short palindromic repeat 1 conserved sequence 1 controlled study 1 crop production 1 double stranded DNA break 1 gene editing 1 gene knockout 1 gene sequence 1 gene structure 1 genetic analysis 1 genetics 1 genome editing 1 guide RNA 1 hexaploidy 1