Animals 1 Anxiety 1 Article 1 Behavior, Animal 1 Cognition 1 Depression 1 Depression-like behavior 1 Emotions 1 Handling 1 Rats 1 SIRT1 1 Social Isolation 1 Social isolation 1 Stress 1 Stress, Psychological 1 adolescent 1 adolescent development 1 animal 1 animal behavior 1 animal experiment 1 animal tissue 1 anxiety 1 behavior change 1 brain analysis 1 cognition 1 cognitive development 1 controlled study 1 depression 1 dorsal raphe nucleus 1 emotion 1