human 204 Article 192 real time polymerase chain reaction 181 Humans 179 controlled study 160 genetics 126 male 115 female 107 adult 98 nonhuman 92 gene expression 83 Male 81 metabolism 78 Female 77 unclassified drug 75 Real- time 71 middle aged 62 major clinical study 61 Middle Aged 58 protein expression 56 upregulation 56 real time reverse transcription polymerase chain reaction 51 Real-Time Polymerase Chain Reaction 49 gene expression regulation 49 human tissue 48 Western blotting 47 human cell 46 pathology 45 Real time systems 43 Animals 42