real time polymerase chain reaction 181 human 138 Article 137 Humans 122 controlled study 112 genetics 97 male 79 female 74 nonhuman 69 adult 65 metabolism 64 gene expression 63 Female 57 Male 56 unclassified drug 51 Real-Time Polymerase Chain Reaction 49 protein expression 46 middle aged 43 upregulation 41 Middle Aged 40 Western blotting 39 major clinical study 39 gene expression regulation 36 messenger RNA 33 human cell 32 human tissue 31 pathology 31 animal experiment 29 Animals 27 down regulation 27