Article DNA replication origin DNA supercoiling DNA topoisomerase RNA sequencing Sporulation Streptomyces Streptomyces coelicolor Topoisomerases Transcription factors Transcriptional regulation Two-component system agar gel electrophoresis chromosome controlled study drug manufacture gene gene activity gene control gene expression gene induction genetic background genetic transcription genotype nonhuman phase contrast microscopy phenotype room temperature soil microflora sporogenesis