Apoptosis Chemotaxis Cotinine Craniofacial regeneration Extracellular Traps Flow Cytometry Gene Expression Humans Lyophilisation Neutrophils Nicotine Platelet concentrate Platelet-rich fibrin Reactive Oxygen Species Real-Time Polymerase Chain Reaction Smoke Smoking Thiocyanates Tissue engineering apoptosis chemotaxis cigarette smoke cotinine drug effect extracellular trap flow cytometry gene expression human inflammation metabolism