Real-Time Polarization-Resolved Imaging of Living Tissues Based on Two-Photon Excitation Spinning-Disk Confocal Microscopy

by Ai Goto, Ai Goto, Kohei Otomo, Kohei Otomo, Tomomi Nemoto, Tomomi Nemoto
Published 2019-04-01

In vivo two-photon microscopic observation and ablation in deeper brain regions realized by modifications of excitation beam diameter and immersion liquid.

by Kazushi Yamaguchi, Ryoji Kitamura, Ryosuke Kawakami, Kohei Otomo, Tomomi Nemoto
Published 2020-01-01

Protocol for constructing an extensive cranial window utilizing a PEO-CYTOP nanosheet for in vivo wide-field imaging of the mouse brain

by Taiga Takahashi, Hong Zhang, Kohei Otomo, Yosuke Okamura, Tomomi Nemoto
Published 2021-06-01

Nanosheet wrapping-assisted coverslip-free imaging for looking deeper into a tissue at high resolution.

by Hong Zhang, Kenji Yarinome, Ryosuke Kawakami, Kohei Otomo, Tomomi Nemoto, Yosuke Okamura
Published 2020-01-01

PEO-CYTOP Fluoropolymer Nanosheets as a Novel Open-Skull Window for Imaging of the Living Mouse Brain

by Taiga Takahashi, Hong Zhang, Ryosuke Kawakami, Kenji Yarinome, Masakazu Agetsuma, Junichi Nabekura, Kohei Otomo, Yosuke Okamura, Tomomi Nemoto
Published 2020-10-01

3DeeCellTracker, a deep learning-based pipeline for segmenting and tracking cells in 3D time lapse images

by Chentao Wen, Takuya Miura, Venkatakaushik Voleti, Kazushi Yamaguchi, Motosuke Tsutsumi, Kei Yamamoto, Kohei Otomo, Yukako Fujie, Takayuki Teramoto, Takeshi Ishihara, Kazuhiro Aoki, Tomomi Nemoto, Elizabeth MC Hillman, Koutarou D Kimura
Published 2021-03-01